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Organotypic slice culture of the hypothalamic paraventricular nucleus of rat
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Eun Seong | - |
dc.contributor.author | Lee, So Yeong | - |
dc.contributor.author | Park, Jae-Yong | - |
dc.contributor.author | Hong, Seong-Geun | - |
dc.contributor.author | Ryu, Pan Dong | - |
dc.date.accessioned | 2009-08-11T22:45:37Z | - |
dc.date.available | 2009-08-11T22:45:37Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | J Vet Sci 2007, 8, 15-20 | en |
dc.identifier.issn | 1229-845X | - |
dc.identifier.uri | http://www.vetsci.org/2007/abstract/15a.html | - |
dc.identifier.uri | https://hdl.handle.net/10371/6966 | - |
dc.description.abstract | Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro. | en |
dc.description.sponsorship | The authors thank Dr. Seung Yub Shin, Mr. Tae Hee Han,
and Mr. Jian Hua Yang for their technical assistance. This work was supported by a grant (R01-2002-000-00128-0) from the Basic Research Program of the Korea Science and Engineering Foundation, and was performed as a portion of the Masters degree of the late Dr. Eun Seong Cho. | en |
dc.language.iso | en | en |
dc.publisher | 대한수의학회 = The Korean Society of Veterinary Science | en |
dc.subject | electrophysiology | en |
dc.subject | green fluorescent proteingreen fluorescent protein | en |
dc.subject | immunocytochemistry | en |
dc.subject | organotypic slice culture | en |
dc.subject | paraventricular nucleus | en |
dc.title | Organotypic slice culture of the hypothalamic paraventricular nucleus of rat | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 조은성 | - |
dc.contributor.AlternativeAuthor | 이소영 | - |
dc.contributor.AlternativeAuthor | 박재용 | - |
dc.contributor.AlternativeAuthor | 홍성근 | - |
dc.contributor.AlternativeAuthor | 류판동 | - |
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