S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
A TGF-beta-induced gene, betaig-h3, is crucial for the apoptotic disappearance of the medial edge epithelium in palate fusion.
- Choi, Kang-Young; Kim, Hyun-Jung; Cho, Byung-Chae; Kim, In-San; Kim, Hyun-Jung; Ryoo, Hyun-Mo
- Issue Date
- Journal of Cellular Biochemistry 107:818–825
- TGF-b3, TbR-I, and TGF-b-activated Smad2 has been suggested to be a series of signaling molecules for secondary palate fusion. In this
article, we show that a gene induced by TGF-b, big-h3, is coincidentally expressed with TGF-b3 in medial edge epithelial (MEE) cells
undergoing apoptosis during normal palatal fusion. big-h3 was also highly expressed in the areas of post-weaning mammary gland cells and
developing phalangeal joints in which TGF-b3 or BMP-4-induced apoptosis occurs, respectively. Blocking of big-h3 expression in E12.5
embryos with antisense oligodeoxynucleotides (ODN) resulted in cleft of the secondary palate in 84% of the treated mice that were born.
Moreover, the antisense ODN treatment resulted in a failure of apoptosis in the MEE between palatal shelves in physical contact in organ
culture. We conclude that big-h3 expression in the MEE is stimulated by TGF-b3, causes cell death, and consequently results in complete
fusion of the apposed palatal shelves.
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