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Identification of genes modulated by high extracellular calcium in coculuture of mouse osteoblasts and bone marrow cells by oligo chip assay
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Gwan-Shik | - |
dc.contributor.author | Kim, Hyung-Keun | - |
dc.contributor.author | Song, Mina | - |
dc.contributor.author | Jun, Ji-Hae | - |
dc.contributor.author | Woo, Kyung Mi | - |
dc.contributor.author | Baek, Jeong Hwa | - |
dc.date.accessioned | 2010-09-06T03:15:56Z | - |
dc.date.available | 2010-09-06T03:15:56Z | - |
dc.date.issued | 2006-05 | - |
dc.identifier.citation | International Journal of Oral Biology. 31:53-65 | en |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://hdl.handle.net/10371/69743 | - |
dc.description.abstract | Calcium concentration in the bone resorption lacunae is
high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone metabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)2vitaminD3 (VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM [Ca2+]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expression of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high extracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated; slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated; s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracellular calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes. | en |
dc.description.sponsorship | 본 연구는 보건복지부 보건의료기술진흥사업의 지원에
의하여 이루어진 것임(03-PJ1-PG3-20500-0013). | en |
dc.language.iso | ko | en |
dc.publisher | Korean Academy of Oral Biology | en |
dc.subject | high extracellular calcium | en |
dc.subject | coculture of osteo-blasts and bone marrow cells | en |
dc.subject | osteoclast | en |
dc.subject | oligo chip assay | en |
dc.title | Identification of genes modulated by high extracellular calcium in coculuture of mouse osteoblasts and bone marrow cells by oligo chip assay | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김관식 | - |
dc.contributor.AlternativeAuthor | 김형근 | - |
dc.contributor.AlternativeAuthor | 송미나 | - |
dc.contributor.AlternativeAuthor | 전지혜 | - |
dc.contributor.AlternativeAuthor | 우경미 | - |
dc.contributor.AlternativeAuthor | 백정화 | - |
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