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Biomimetic Approach on Human Periodontal Ligament Cells Using Synthetic Oligopeptides

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dc.contributor.authorKim, Tae-Il-
dc.contributor.authorJang, Jun-Hyeog-
dc.contributor.authorLee, Yong-Moo-
dc.contributor.authorRhyu, In-Chul-
dc.contributor.authorChung, Chong-Pyoung-
dc.contributor.authorHan, Soo-Boo-
dc.contributor.authorChoi, Sang-Mook-
dc.contributor.authorKu, Young-
dc.date.accessioned2010-09-12T23:48:10Z-
dc.date.available2010-09-12T23:48:10Z-
dc.date.issued2004-07-
dc.identifier.citationJ Periodontol. 2004;75(7):925-932en
dc.identifier.issn0022-3492-
dc.identifier.urihttps://hdl.handle.net/10371/69803-
dc.description.abstractBACKGROUND: Periodontal ligament (PDL) cells, connecting root cementum with alveolar bone, are important for periodontal wound healing. In order to obtain a predictable periodontal regeneration, selective adhesion and proliferation of PDL cells are essential. The purpose of this study was to investigate the effects of synthetic peptides mimicking cell-binding domain of fibronectin (FN) on human PDL cells. METHODS: Two types of oligopeptides, Gly3-Pro-His-Ser-Arg-Asn-Gly6-Arg-Gly-Asp-Gly (G3PHSRNG6RGDG) and Gly3-His-Pro-Asn-Arg-Ser-Gly6-Arg-Gly-Asp-Gly (G3HPNRSG6RGDG), were constructed using a solid-phase peptide synthesizer. Fibronectin type III ninth to tenth domain (FN III 9-10) and Arg-Gly-Asp-Ser (RGDS) were prepared for comparison with the effects of synthetic peptides. These peptides were coated onto 96-well cell culture plates with 0.001 approximately 100 microM concentrations. Cultured human PDL cells were then applied to the peptide-coated wells at a density of 1 x 10(4)/well. After 1 hour incubation at 37 degrees C, adhered cells were fixed, stained, and examined by phase contrast microscopy for cell spreading assay. Attached PDL cells were solubilized with 2% sodium dodecyl sulfate (SDS) for the cell attachment assay by measuring absorbance at 595 nm in microplate reader. Western blot analysis was performed to determine extracellular signal-regulated kinase (ERK1/2) activity. RESULTS: Cell attachment and spreading assays revealed that G3PHSRNG6RGDG (> or = 10 microM) possesses similar adhesive behavior to FN III 9-10. G3PHSRNG6RGDG showed a comparable ERK1/2 activity when compared to FN III 9-10. CONCLUSIONS: G3PHSRNG6RGDG enhanced an attachment and spreading of human PDL cells thereby increasing ERK1/2 activity. Taken together, it is anticipated that this peptide might be a potential tool for arranging a biologically attractive environment for PDL cells, which would enhance periodontal regeneration efficacy.en
dc.description.sponsorshipThis work was supported by a grant from the Korea
Health 21 R&D Project, Ministry of Health and
Welfare, Republic of Korea (02-PJ3-PG6-EV11-0002).
en
dc.language.isoenen
dc.publisherAmerican Academy of Periodontologyen
dc.titleBiomimetic Approach on Human Periodontal Ligament Cells Using Synthetic Oligopeptidesen
dc.typeArticleen
dc.contributor.AlternativeAuthor김태일-
dc.contributor.AlternativeAuthor장준혁-
dc.contributor.AlternativeAuthor이용무-
dc.contributor.AlternativeAuthor류인철-
dc.contributor.AlternativeAuthor정종평-
dc.contributor.AlternativeAuthor한수부-
dc.contributor.AlternativeAuthor최상묵-
dc.contributor.AlternativeAuthor구영-
dc.identifier.doi10.1902/jop.2004.75.7.925-
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