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Biomimetic Approach on Human Periodontal Ligament Cells Using Synthetic Oligopeptides
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Tae-Il | - |
dc.contributor.author | Jang, Jun-Hyeog | - |
dc.contributor.author | Lee, Yong-Moo | - |
dc.contributor.author | Rhyu, In-Chul | - |
dc.contributor.author | Chung, Chong-Pyoung | - |
dc.contributor.author | Han, Soo-Boo | - |
dc.contributor.author | Choi, Sang-Mook | - |
dc.contributor.author | Ku, Young | - |
dc.date.accessioned | 2010-09-12T23:48:10Z | - |
dc.date.available | 2010-09-12T23:48:10Z | - |
dc.date.issued | 2004-07 | - |
dc.identifier.citation | J Periodontol. 2004;75(7):925-932 | en |
dc.identifier.issn | 0022-3492 | - |
dc.identifier.uri | https://hdl.handle.net/10371/69803 | - |
dc.description.abstract | BACKGROUND: Periodontal ligament (PDL) cells, connecting root cementum with alveolar bone, are important for periodontal wound healing. In order to obtain a predictable periodontal regeneration, selective adhesion and proliferation of PDL cells are essential. The purpose of this study was to investigate the effects of synthetic peptides mimicking cell-binding domain of fibronectin (FN) on human PDL cells. METHODS: Two types of oligopeptides, Gly3-Pro-His-Ser-Arg-Asn-Gly6-Arg-Gly-Asp-Gly (G3PHSRNG6RGDG) and Gly3-His-Pro-Asn-Arg-Ser-Gly6-Arg-Gly-Asp-Gly (G3HPNRSG6RGDG), were constructed using a solid-phase peptide synthesizer. Fibronectin type III ninth to tenth domain (FN III 9-10) and Arg-Gly-Asp-Ser (RGDS) were prepared for comparison with the effects of synthetic peptides. These peptides were coated onto 96-well cell culture plates with 0.001 approximately 100 microM concentrations. Cultured human PDL cells were then applied to the peptide-coated wells at a density of 1 x 10(4)/well. After 1 hour incubation at 37 degrees C, adhered cells were fixed, stained, and examined by phase contrast microscopy for cell spreading assay. Attached PDL cells were solubilized with 2% sodium dodecyl sulfate (SDS) for the cell attachment assay by measuring absorbance at 595 nm in microplate reader. Western blot analysis was performed to determine extracellular signal-regulated kinase (ERK1/2) activity. RESULTS: Cell attachment and spreading assays revealed that G3PHSRNG6RGDG (> or = 10 microM) possesses similar adhesive behavior to FN III 9-10. G3PHSRNG6RGDG showed a comparable ERK1/2 activity when compared to FN III 9-10. CONCLUSIONS: G3PHSRNG6RGDG enhanced an attachment and spreading of human PDL cells thereby increasing ERK1/2 activity. Taken together, it is anticipated that this peptide might be a potential tool for arranging a biologically attractive environment for PDL cells, which would enhance periodontal regeneration efficacy. | en |
dc.description.sponsorship | This work was supported by a grant from the Korea
Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (02-PJ3-PG6-EV11-0002). | en |
dc.language.iso | en | en |
dc.publisher | American Academy of Periodontology | en |
dc.title | Biomimetic Approach on Human Periodontal Ligament Cells Using Synthetic Oligopeptides | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김태일 | - |
dc.contributor.AlternativeAuthor | 장준혁 | - |
dc.contributor.AlternativeAuthor | 이용무 | - |
dc.contributor.AlternativeAuthor | 류인철 | - |
dc.contributor.AlternativeAuthor | 정종평 | - |
dc.contributor.AlternativeAuthor | 한수부 | - |
dc.contributor.AlternativeAuthor | 최상묵 | - |
dc.contributor.AlternativeAuthor | 구영 | - |
dc.identifier.doi | 10.1902/jop.2004.75.7.925 | - |
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