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Immunoproteomic analysis of capsulate and non-capsulate strains of Lactococcus garvieae

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dc.contributor.authorShin, G.W.-
dc.contributor.authorPalaksha, K.J.-
dc.contributor.authorKim, Y.R.-
dc.contributor.authorNho, S.W.-
dc.contributor.authorCho, J.H.-
dc.contributor.authorHeo, N.E.-
dc.contributor.authorHeo, G.J.-
dc.contributor.authorPark, Se Chang-
dc.contributor.authorJung, T.S.-
dc.date.accessioned2009-08-12T08:17:21Z-
dc.date.available2009-08-12T08:17:21Z-
dc.date.issued2006-08-26-
dc.identifier.citationVet. Microbiol. 119, 205-212en
dc.identifier.issn0378-1135-
dc.identifier.urihttp://hdl.handle.net/10371/7045-
dc.description.abstractA comparative immunoproteomic study was carried out to investigate the immunogenicity of capsulate (KG9408) and non-capsulate (NSS9310) strains of Lactococcus garvieae. Immunoblot assays, following two-dimensional gel electrophoresis (2-DE) for L. garvieae strains, revealed a significant difference between anti-capsulate and anti-non-capsulate rabbit sera with respect to the number and antigenicity of antigenic spots. Anti-capsulate and anti-non-capsulate rabbit sera reacted with an average of 72 and 127 antigenic spots, respectively. The strong reaction of anti-non-capsulate sera with elongation factor (EF)-G and -Tu, and GMP synthase, of the L. garvieae strains identifies these as specific major antigens. This study clearly demonstrates the differences in 2-DE immunoblot profiles between the capsulate and non-capsulate strains of L. garvieae. These differences may be the reason for variations in immunogenicity between capsulate and non-capsulate strains. Glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, arginine deaminase and ornithine carbamoyltransferase were identified from the 2-DE immunoblot profiles of both strains. Therefore, these common antigens are potential markers for the development of vaccines against L. garvieae, irrespective of strain. Immunoproteomics, a powerful tool for studying antigens at the proteomic level, allowed a comparative investigation of the immunogenicity of capsulate and non-capsulate strains of L. garvieae for vaccine development.en
dc.description.sponsorshipThe rabbit anti-KG9408 and anti-NSS9310 sera and the L. garvieae strains KG9408 and NSS9310 strain used in this study was kindly provided by Dr. Yoshida (Department of Fisheries, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan). This work was supported by a grant (R01-2001-000-00242-0) from the Basic Research Program of the Korea Science & Engineering Foundation.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectLactococcus garvieaeen
dc.subjectImmunoproteomicsen
dc.subjectCapsuleen
dc.subjectAntigenic profilesen
dc.titleImmunoproteomic analysis of capsulate and non-capsulate strains of Lactococcus garvieaeen
dc.typeArticleen
dc.contributor.AlternativeAuthor박세창-
dc.identifier.doi10.1016/j.vetmic.2006.08.021-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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