각종 질환에 있어서의 HLA빈도에 관한 연구

Abstract

The purpose of this study is to investigate distribution of the HLA antigens among Korean population and to elucidate any correlation between the HLA antigens and various human diseases. The subjects for this study consisted of 85 normal healthy persons, 28 patients with stomach cancer, 24 with liver cancer, and 32 with pulmonary tuberculosis. The diagnosis of cancer was confirmed either by the radiological, endo scopic, serologic or histopathologic findings, and patients with pulmonary tuberculosis included only those who had moderate advanced or far advanced pulmonary tuberculosis. The detection of HLA antigens was carried out with the micortoxicity test devised by Tcrasaki, the lymphocytes being seperated from peripheral blood by density-gradient centrifugation in the Ficoll-Hypaque solution. The standard HLA antiserum used in this study was the T-serics antiserum provided by the Beringwerke company, West Germany. The antiserum included HLA-A 1, 2, 3, 9,10.11, and HLA-B 5,7,8,12. The positive and negative control sera were also used. The observed data are summarized as follows: 1. The detection rate of the HLA antigens was essentially the same among normal healthy persons. and patients with stomach cancer, liver cancer, and pulmonary tuberculosis. 2. In the normal control group, HLA·A2 and A3 were most frequently detected in the first segregation series, and HLA-B 12 and B 7 in the second segregation series, respectively. 3. In the group of stomach cancer, the most frequent HLA antigens were HLA-A2 and AIO in the first segregation series, and IlLA-B5 and B8 in the second segregation series, respectively. As compared with normal control group, IlLA-AIO was slightly increased and HLA-BI2 were markedly reduced in frequency among the patients with stomach cancer. 4. In the group of the liver cancer, the most frequent HLA antigens were HLA-A2 and AIO in the first segregation series, and HLA-B8 and BI2 in the second segregation series. As compared with the normal control group, the HLA-B7 was markedly reduced in frequency in the patients with liver cancer. 5. In the patients with pulmonary tuberculosis, the most frequent HLA autigens were HLA-A2 and AIO in the first segregation series, and HLA-B5 and Bl2 in the second segregation series. As compared with the normal control group, HLA-B7 was slightly reduced and HLA-BI2 was markedly reduced in frequency in the patients with pulmonary tubeculosis.