Browse
S-Space
College of Medicine/School of Medicine (의과대학/대학원)
Dept. of Medicine (의학과)
The Seoul Journal of Medicine
The Seoul Journal of Medicine Vol. 23 No.1 (1982)
심장근 근장그물에서의 Ca++과 Na+에 의한 Ca++유리작용 : The Ca*-and Nat-Induced Ca" Release from Isolated Cardiac Sarcoplasmic Reticulum
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김해원 | - |
dc.contributor.author | 김명석 | - |
dc.contributor.author | 박찬웅 | - |
dc.date.accessioned | 2009-08-15T12:16:46Z | - |
dc.date.available | 2009-08-15T12:16:46Z | - |
dc.date.issued | 1982-03 | - |
dc.identifier.citation | Seoul J Med, Vol.23 No.1, pp. 7-16 | - |
dc.identifier.issn | 0582-6802 | - |
dc.identifier.uri | https://hdl.handle.net/10371/7234 | - |
dc.description.abstract | The Ca't-and Nat-induced Ca" release was studied
in sarcoplasmic reticulum isolated from porcine ventricles by milipore filtration method using radioisot· ope "sCa. The isolated cardiac sarcoplasmic reticulum took up calcium maximally 40 to 50 min of incubation in the presence of 50mM inorganic phosphate. After reaching the maximal uptake, the spontaneous release of calcium was observed at high concentrations of CaCI, (above l50"M) in the medium, at the low concentrations of CaC!" however, the amount of calcium taken up was retained for prolonged period. Unidirectional calcium efflux, calulated from mea' surements of influx of tracer 4fiCa at steady state calcium uptake, was stimulated by increasing external remaining calcium concentrations in the range of 1 to 201,M. The initial calcium efflux showed saturation kinetics with the apparent KCa uf 7/,M and a maximal calcium efflux of 180 nmol of calcium/mg protein/min. In the absence of fiDmM inorganic phosphate, rna' ximal calcium binding by isolated cardiac sarcoplasmic reticulum in the free calcium concentrations of 5 x 10-8 to 5 x 1O-5M was 40 nmol/mg protein. Calcium release was not observed by 1 to 10mM Na" in the medium containing 0.5mM EGTA to prevent sarcoplasmic reticulum from rebinding the released Caito Tbe changes in Na'/K+ ratio also did not influence the calcium binding or release. From the experiment, the Ca"-induced Ca" release from cardiac sarcoplasmic reticulum may be one of the calcium release mechanisms, and the released Ca" could initiate the cardiac contraction In the physiclogic conditions. The NaI itself and changes in Na" /K I ratio might he no influence on calcium hinding and release mechanism in cardiac sarcoplasmic reticulum. | - |
dc.language.iso | ko | - |
dc.publisher | 서울대학교 의과대학 | - |
dc.title | 심장근 근장그물에서의 Ca++과 Na+에 의한 Ca++유리작용 | - |
dc.title.alternative | The Ca*-and Nat-Induced Ca" Release from Isolated Cardiac Sarcoplasmic Reticulum | - |
dc.type | SNU Journal | - |
dc.contributor.AlternativeAuthor | Kim, Hae Won | - |
dc.contributor.AlternativeAuthor | Kim, Myung Suk | - |
dc.contributor.AlternativeAuthor | Park, Chan Woong | - |
dc.citation.journaltitle | 서울 의대 잡지 | - |
dc.citation.journaltitle | 서울 의대 학술지 | - |
dc.citation.journaltitle | Seoul Journal of Medicine | - |
dc.citation.endpage | 16 | - |
dc.citation.number | 1 | - |
dc.citation.pages | 7-16 | - |
dc.citation.startpage | 7 | - |
dc.citation.volume | 23 | - |
- Appears in Collections:
- College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 23 No.1 (1982)
- Files in This Item:
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.