Publications
Detailed Information
The enhanced reactivity of endogenous biotin-like molecules by antigen retrieval procedures and signal amplification with tyramine
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Seok Hyung | - |
dc.contributor.author | Jung, Kyeong Cheon | - |
dc.contributor.author | Shin, Young Kee | - |
dc.contributor.author | Lee, Kyung Mee | - |
dc.contributor.author | Park, Young S. | - |
dc.contributor.author | Choi, Yoon La | - |
dc.contributor.author | Oh, Kwon Ik | - |
dc.contributor.author | Kim, Min Kyung | - |
dc.contributor.author | Chung, Doo Hyun | - |
dc.contributor.author | Song, Hyung Geun | - |
dc.contributor.author | Park, Seong Hoe | - |
dc.date.accessioned | 2011-07-22T02:44:59Z | - |
dc.date.available | 2011-07-22T02:44:59Z | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Histochem J; Vol.34, No.3-4; pp.97–103 | en |
dc.identifier.issn | 1573-6865 | - |
dc.identifier.uri | https://hdl.handle.net/10371/73497 | - |
dc.description.abstract | In diagnostic pathology and immunocytochemical research, immunohistochemical techniques using the streptavidin–biotin–peroxidase system have played an extremely valuable role. This system, based on the high affinity of streptavidin for biotin, may, however, provoke false positive results because of endogenous streptavidin-binding sites in human tissues.With the advent of the
antigen retrieval procedure and signal amplification method, this problem can be serious enough to cause mistakes in interpreting immunohistochemical staining results. Therefore, we examined the distribution of endogenous biotin-like molecules in various human tissues and the influence of various antigen retrieval procedures with or without signal amplification using biotinylated tyramine to reveal these biotin-like activities. We observed that endogenous biotin-like molecules were present in a wide range of tissues, and their activity was markedly enhanced by employing antigen retrieval procedures or signal amplification. Furthermore, the extent to which the activity of endogenous biotin-like activities was enhanced depended on the kinds of antigen retrieval procedures and signal amplification employed. Pressure cooking and tyramine amplification with microwave heating showed the highest activities. These results show that the antigen retrieval procedures and signal amplification with tyramine can enhance the activity of endogenous biotin or biotin-like molecules as well as antigenicity, which can be a pitfall in the interpretation of immunohistochemical data. | en |
dc.language.iso | en | en |
dc.publisher | Kluwer Academic Publishers | en |
dc.title | The enhanced reactivity of endogenous biotin-like molecules by antigen retrieval procedures and signal amplification with tyramine | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김석형 | - |
dc.contributor.AlternativeAuthor | 정경천 | - |
dc.contributor.AlternativeAuthor | 신영기 | - |
dc.contributor.AlternativeAuthor | 이경미 | - |
dc.contributor.AlternativeAuthor | 최윤라 | - |
dc.contributor.AlternativeAuthor | 오권익 | - |
dc.contributor.AlternativeAuthor | 김민경 | - |
dc.contributor.AlternativeAuthor | 정두현 | - |
dc.contributor.AlternativeAuthor | 송형근 | - |
dc.contributor.AlternativeAuthor | 박성회 | - |
dc.identifier.doi | 10.1023/A:1020954611464 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.