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Msx2 mediates the inhibitory action of tnf-α on osteoblast differentiation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hye-Lim | - |
dc.contributor.author | Yi, TacGhee | - |
dc.contributor.author | Woo, Kyung Mi | - |
dc.contributor.author | Ryoo, Hyun-Mo | - |
dc.contributor.author | Kim, Gwan-Shik | - |
dc.contributor.author | Baek, Jeong-Hwa | - |
dc.date.accessioned | 2011-10-14T04:34:23Z | - |
dc.date.available | 2011-10-14T04:34:23Z | - |
dc.date.issued | 2010-06 | - |
dc.identifier.citation | Experimental and Molecular Medicine 2010;42:437-445 | en |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://hdl.handle.net/10371/74146 | - |
dc.description.abstract | TNF-α, a proinflammatory cytokine, inhibits osteoblast differentiation under diverse inflammatory conditions; however, the underlying mechanisms in terms of the TNF-α signaling pathway remain unclear. In this study, we examined the role of Msx2 in TNF-α-mediated inhibition of alkaline phosphatase (ALP) expression and the signaling pathways involved. TNF-α down-regulated ALP expression induced by bone morphogenetic protein 2 (BMP2) in C2C12 and Runx2-/- calvarial cells. Over-expression of Msx2 suppressed BMP2-induced ALP expression. Furthermore, TNF-α induced Msx2 expression, and the knockdown of Msx2 by small interfering RNAs rescued ALP expression, which was inhibited by TNF-α. TNF-α activated the NF-κB and the JNK pathways. Inhibition of NF-κB or JNK activation reduced the inhibitory effect of TNF-α on ALP expression, whereas TNF-α-induced Msx2 expression was only suppressed by the inhibition of the NF-κB pathway. Taken together, these results indicate that Msx2 mediates the inhibitory action of TNF-α on BMP2-regulated osteoblast differentiation and that the TNF-α-activated NF-κB pathway is responsible for Msx2 induction. | en |
dc.description.sponsorship | This work was supported by the Korea Science and Engineering Foundation Grant 10646010002-06N4601-00210 funded by the Korean government (MOST) and by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-000-E00093, KRF-2007-000-E00073). | en |
dc.language.iso | en | en |
dc.publisher | Korean Society of Medical Biochemistry and Molecular Biology | en |
dc.subject | alkaline phosphatase | en |
dc.subject | bone morphogenetic protein 2 | en |
dc.subject | cell differentiation | en |
dc.subject | Msx2 protein | en |
dc.subject | NF-κB | en |
dc.subject | osteoblasts | en |
dc.subject | tumor necrosis factor-α | en |
dc.title | Msx2 mediates the inhibitory action of tnf-α on osteoblast differentiation | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이혜림 | - |
dc.contributor.AlternativeAuthor | 이택기 | - |
dc.contributor.AlternativeAuthor | 우경미 | - |
dc.contributor.AlternativeAuthor | 류현모 | - |
dc.contributor.AlternativeAuthor | 김관식 | - |
dc.contributor.AlternativeAuthor | 백정화 | - |
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