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In vitro investigation of anodization and CaP deposited titanium surface using MG63 osteoblast-like cells

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dc.contributor.authorLee, J. M.-
dc.contributor.authorLee, J. I.-
dc.contributor.authorLim, Y. J.-
dc.date.accessioned2011-10-14T04:38:19Z-
dc.date.available2011-10-14T04:38:19Z-
dc.date.issued2010-03-
dc.identifier.citationApplied Surface Science 2010;256:3086-3092en
dc.identifier.issn0169-4332-
dc.identifier.urihttps://hdl.handle.net/10371/74147-
dc.description.abstractThe aim of the present study was to investigate surface characteristics in four different titanium surfaces (AN: anodized at 270 V; AN-CaP: anodic oxidation and CaP deposited; SLA: sandblasted and acid etched; MA: machined) and to evaluate biological behaviors such as cell adhesion, cell proliferation, cytoskeletal organization, and osteogenic protein expression of MG63 osteoblast-like cells at the early stage. Surface analysis was performed using scanning electron microscopy, thin-film X-ray diffractometry, and a confocal laser scanning microscope. In order to evaluate cellular responses, MG63 osteoblast-like cells were used. The cell viability was evaluated by MTT assay. Immunofluorescent analyses of actin, type I collagen, osteonectin and osteocalcin were performed. The anodized and CaP deposited specimen showed homogeneously distributed CaP particles around micropores and exhibited anatase type oxides, titanium, and HA crystalline structures. This experiment suggests that CaP particles on the anodic oxidation surface affect cellular attachment and spreading. When designing an in vitro biological study for CaP coated titanium, it must be taken into account that preincubation in medium prior to cell seeding and the cell culture medium may affect the CaP coatings. All these observations illustrate the importance of the experimental conditions and the physicochemical parameters of the CaP coating. It is considered that further evaluations such as long-term in vitro cellular assays and in vivo experiments should be necessary to figure out the effect of CaP deposition to biological responses.en
dc.description.sponsorshipThis work was supported by grant no 04-2006-0009 from the Seoul National University Dental Hospital Research Fund, Seoul National University Dental Hospital, Seoul 110-768.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectCaP electron beam depositionen
dc.subjectSurface characteristicsen
dc.subjectMG63 osteoblast-like cellsen
dc.subjectImmunofluorescent analysesen
dc.subjectOsteogenic protein expressionen
dc.titleIn vitro investigation of anodization and CaP deposited titanium surface using MG63 osteoblast-like cellsen
dc.typeArticleen
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