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Ex vivo bone morphogenetic protein-2 gene delivery using gingival fibroblasts promotes bone regeneration in rats

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dc.contributor.authorShin, Joong-Ho-
dc.contributor.authorKim, Kyoung-Hwa-
dc.contributor.authorKim, Su-Hwan-
dc.contributor.authorKoo, Ki-Tae-
dc.contributor.authorKim, Tae-Il-
dc.contributor.authorSeol, Yang-Jo-
dc.contributor.authorKu, Young-
dc.contributor.authorRhyu, In-Chul-
dc.contributor.authorChung, Chong-Pyoung-
dc.contributor.authorLee, Yong-Moo-
dc.date.accessioned2011-10-17-
dc.date.available2011-10-17-
dc.date.issued2010-02-
dc.identifier.citationJ Clin Periodontol 2010;37:305-311en
dc.identifier.issn0303-6979-
dc.identifier.urihttps://hdl.handle.net/10371/74181-
dc.description.abstractAim: The aim of the present study was to investigate bone regeneration following ex vivo bone morphogenetic protein-2 (BMP-2) gene delivery using human gingival fibroblasts (HGFs) in rat calvarial defects. Materials and Methods: An 8 mm craniotomy defect was created in Sprague-Dawley rats. The animals were divided into four groups: (1) non-grafted group, the defect was left empty; (2) collagen matrix group, the defect was filled with collagen matrix only; (3) HGF group, the defect was filled with non-transduced HGFs on collagen matrix; (4) BMP-2/HGF group, the defect was filled with BMP-2 gene-transduced HGFs on collagen matrix. Animals were sacrificed at 2 and 4 weeks after surgery, and micro-computed tomographic and histologic observations were performed. Results: The BMP-2/HGF group showed promoted osseous healing of calvarial defects, as compared with the other groups. At both 2 and 4 weeks, regenerated bone area was significantly greater in the BMP-2/HGF group than the other three groups. Quite a few number of transplanted HGFs were observed within the regenerated bone tissues. Conclusions: The results of this study suggest that ex vivo BMP-2 gene delivery induces prominent bone regeneration in vivo and HGFs may be useful as target cells for ex vivo gene therapy.en
dc.description.sponsorshipThis work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. M10646010003-08N4601-00310).en
dc.language.isoenen
dc.publisherJohn Wiley and Sonsen
dc.subjectbone morphogenetic protein-2 (BMP-2) geneen
dc.subjectbone regenerationen
dc.subjectex vivo gene therapyen
dc.subjectgingival fibroblastsen
dc.subjecttransplantationen
dc.titleEx vivo bone morphogenetic protein-2 gene delivery using gingival fibroblasts promotes bone regeneration in ratsen
dc.typeArticleen
dc.contributor.AlternativeAuthor신중호-
dc.contributor.AlternativeAuthor김경화-
dc.contributor.AlternativeAuthor김수환-
dc.contributor.AlternativeAuthor구기태-
dc.contributor.AlternativeAuthor김태일-
dc.contributor.AlternativeAuthor설양조-
dc.contributor.AlternativeAuthor구영-
dc.contributor.AlternativeAuthor류인철-
dc.contributor.AlternativeAuthor정종평-
dc.contributor.AlternativeAuthor이용무-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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