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Methylation status of the O6-methylguanine-deoxyribonucleic acid methyltransferase gene promoter in World Health Organization Grade III gliomas

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Authors
Yang, Seung-Heon; Kim, Yong Hwy; Kim, Jin Wook; Park, Chul-Kee; Park, Sung-Hye; Jung, Hee-Won
Issue Date
2009-10
Publisher
The Korean Neurosurgical Society
Citation
J Korean Neurosurg Soc. 46:385–388,2009
Keywords
MGMT gene promoterMethylation1p/19qOligodendrogliomaMethylation-specific PCR
Abstract
Objective: We analyzed the methylation status of the O6-methylguanine-DNA methyltransferase (MGMT) gene promoter in World Health Organization (WHO) grade III gliomas in association with other molecular markers to evaluate their prevalence.
Methods: The samples of a total of 36 newly WHO grade III glioma patients including 19 anaplastic oligodendrogliomas (AO), 7 anaplastic oligoastrocytomas (AOA), and 10 anaplastic astrocytomas (AA) were analyzed. The methylation status of the MGMT gene promoter was confirmed by methylation-specific polymerase chain reaction. The 1p/19q chromosomal deletion status and EGFR amplification were assessed by Fluorescence In-Situ Hybridization. MGMT, EGFR, EGFRvIII, and p53 expression were analyzed by immunohistochemical staining.
Results: The MGMT gene promoter was methylated in 32 (88.9%) and unmethylated in 4 (11.2%). Among them, all of the AO and AOA had methylated MGMT gene promoter without exception. Significant associations between MGMT gene promoter hypermethylation and 1p/19q deletion was observed (p = 0.003). Other molecular markers failed to show significant associations between MGMT gene promoter statuses.
Conclusion: There was extensive epigenetic silencing of MGMT gene in high grade gliomas with oligodendroglial component. Together with frequent 1p/19q co-deletion in oligodendroglial tumors, this may add plausible explanations supporting the relative favorable prognosis in oligodendroglial tumors compared with pure astrocytic tumors.
ISSN
1598-7876
Language
English
URI
https://hdl.handle.net/10371/74199
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College of Medicine/School of Medicine (의과대학/대학원)Neurosurgery (신경외과학전공)Journal Papers (저널논문_신경외과학전공)
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