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Real-time monitoring of odorant-induced cellular reactions using surface plasmon resonance

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dc.contributor.authorLee, Sang Hun-
dc.contributor.authorKo, Hwi Jin-
dc.contributor.authorPark, Tai Hyun-
dc.date.accessioned2009-08-21T03:26:29Z-
dc.date.available2009-08-21T03:26:29Z-
dc.date.issued2009-06-10-
dc.identifier.citationBiosens. Bioelectron. 25, 55-60en
dc.identifier.issn0956-5663-
dc.identifier.urihttps://hdl.handle.net/10371/7432-
dc.description.abstractSurface plasmon resonance (SPR) is a powerful technique for measuring molecular interaction in real-time. SPR can be used to detect molecule to cell interactions as well as molecule to molecule interactions. In this study, the SPR-based biosensing technique was applied to real-time monitoring of odorant-induced cellular reactions. An olfactory receptor, OR I7, was fused with a rho-tag import sequence at the N-terminus of OR I7, and expressed on the surface of human embryonic kidney (HEK)-293 cells. These cells were then immobilized on a SPR sensor chip. The intensity of the SPR response was linearly dependent on the amount of injected odorant. Among all the aldehyde containing odorants tested, the SPR response was specifically high for octanal, which is the known cognate odorant for the OR I7. This SPR response is believed to have resulted from intracellular signaling triggered by the binding of odorant molecules to the olfactory receptors expressed on the cell surface. This SPR system combined with olfactory receptor-expressed cells provides a new olfactory biosensor system for selective and quantitative detection of volatile compounds.en
dc.description.sponsorshipThis work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. 2009-0080242). The authors wish to acknowledge Dr. Randall R. Reed (Department of Neurosceince, Johns Hopkins University, School of Medicine, Baltimore, Maryland, U.S.A.) for the rho-tag gene; Akihito Yasuoka (Bio-oriented Technology Reseach Advancement Institution, Saitama, Japan) for the pVL-I7 and Paul A. Gargrave (J. Hillis Miller Health Center, Department of Ophthalmology, University of Florida College of Medicine, Gainesville, Florida, U.S.A.) for the B6-30 cell line.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectOlfactory receptor I7en
dc.subjectHuman embryonic kidney (HEK)-293 cellen
dc.subjectOlfactory biosensoren
dc.subjectSurface plasmon resonanceen
dc.titleReal-time monitoring of odorant-induced cellular reactions using surface plasmon resonanceen
dc.typeArticleen
dc.contributor.AlternativeAuthor이상훈-
dc.contributor.AlternativeAuthor고휘진-
dc.contributor.AlternativeAuthor박태현-
dc.identifier.doi10.1016/j.bios.2009.06.007-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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