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Activity-Dependent Neuronal Cell Migration Induced by Electrical Stimulation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Se Hoon | - |
dc.contributor.author | Jun, Sang Beom | - |
dc.contributor.author | Song, Jong Keun | - |
dc.contributor.author | Kim, Sung June | - |
dc.date.accessioned | 2009-08-21T03:33:17Z | - |
dc.date.available | 2009-08-21T03:33:17Z | - |
dc.date.issued | 2009-01 | - |
dc.identifier.citation | Med Biol Eng Comput 47:93-99 | en |
dc.identifier.issn | 0140-0118 (print) | - |
dc.identifier.issn | 1741-0444 (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/7434 | - |
dc.description.abstract | Recently, we found that electrical stimulation
can induce neuronal migration in neural networks cultured for more than 3 weeks on microelectrode arrays. Immunocytochemistry data showed that the aggregation of neurons was related to the emergence of astrocytes in culture. In this study, when neurons were cocultured with astrocytes, electrical stimulation could induce the migration of neuronal cell bodies after only 1 week in culture, while the same stimulation paradigm caused neural necrosis in neuron-only cultures. In addition, the stimulation-induced migration was inhibited by blocking action potentials in neural networks using the voltage-gated sodium channel blocker, tetrodotoxin. Immunocytochemistry was performed to monitor precisely the neuronal migration and count the number of neurons. These results indicate that neuronal migration of cell bodies is dependent on neuronal activity evoked by electrical stimulation and can be enhanced by coculturing with astrocytes. We believe this method can be employed as a means for modifying neural networks and improving the interface between electrodes and neurons. | en |
dc.description.sponsorship | This work was supported by the Korea Science
and Engineering Foundation (KOSEF) through the Nano Bioelectronics and Systems Research Center [Grant No. R11-2000-075- 00002-0 for domestic research and R11- 330 2000-075-01001-0 for international collaboration (June)]. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | Electrical stimulation | en |
dc.subject | Neuronal migration | en |
dc.subject | Microelectrode array | en |
dc.subject | Astrocyte | en |
dc.subject | Neuronal activity | en |
dc.title | Activity-Dependent Neuronal Cell Migration Induced by Electrical Stimulation | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 정세훈 | - |
dc.contributor.AlternativeAuthor | 전상범 | - |
dc.contributor.AlternativeAuthor | 송종근 | - |
dc.contributor.AlternativeAuthor | 김성준 | - |
dc.identifier.doi | 10.1007/s11517-008-0426-8 | - |
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