δ-Hermolysin like Gene of Staphylococcus lugdunensis in Acute Oral Infection Have Partial Homology with δ-Hemolysin Gene of Staphylococcus aureus

Abstract

To investigate the distribution and hemolytic activity of staphylococci in acute oral infection, staphylococci were isolated from the patients with acute oral infection and healthy persons, hemolytic activity was measured on sheep blood agar plates, and DNA-DNA hybridization was preformed with Ghemolysin gene probe of S. aureus under low stringent condition or high stringent condition. The isolation ratio of S. lugdunensis in patients was higher than that of healthy persons. Four strains of S. lugdunensis had Glike hemolytic activity, but two strains did not. In dot blot analysis, S. lugdunensis was hybridized with Ghemolysin probe of S. aureus under low stringent condition, but weakly hybridized with Ghemolysin probe under high stringent condition. These results suggest that S. lugdunensis is an important pathogen in acute oral infection and Ghemolysin gene of S. lugdunensis have partial homology with δ-hemolysin gene of S. aureus.