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Identification of quorum sensing-related regulons in Vibrio vulnificus by two-dimensional gel electrophoresis and differentially displayed reverse transcriptase PCR

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dc.contributor.authorShin, Na-Ri-
dc.contributor.authorLee, Deog Yong-
dc.contributor.authorYoo, Han Sang-
dc.date.accessioned2009-08-21T08:23:48Z-
dc.date.available2009-08-21T08:23:48Z-
dc.date.issued2007-05-14-
dc.identifier.citationFEMS Immunol Med Microbiol 50: 94-103en
dc.identifier.issn0928-8244-
dc.identifier.urihttps://hdl.handle.net/10371/7474-
dc.description.abstractVibrio vulnificus is thought to employ a quorum-sensing system to control the
expression of a global gene. In this study, proteomes and transcriptomes of a lacZ
null mutant, VvSRDZ, and a luxS–smcR double mutant, VvSRDZSR, were
compared with the parent strain, VvAR, by means of two-dimensional gel
electrophoresis (2D-PAGE) and differentially displayed reverse transcriptase PCR
(DDRT-PCR). 2D-PAGE analysis showed that 36 protein spots were differentially
expressed, 14 of which have been identified by peptide-mass fingerprinting. The
expression of eight cellular proteins was repressed by luxS and smcR mutation:
Zn-dependent protease, 6-phosophofructokinase, periplasmic ABC-type Fe31
transport system, deoxyribose-phosphate aldolase, phosphomannomutase,
orotidine-50-phosphate decarboxylase, uridylate kinase, and an unidentified
protein. These proteins are involved in virulence, adaptation to environmental
stress, biosynthesis of LPS, and cell multiplication. Phage shock protein A, a
chemotaxis signal transduction protein, and an uncharacterized low-complexity
protein were activated in the cellular components of the luxS-smcR mutant.
However, only three proteins, of unknown function, were identified in the
extracellular components of the mutants. Analysis of transcriptomes with DDRTPCR
showed that two genes, phosphoribosylformylglycinamidine synthase and
ATP-dependent protease HslVU protease were regulated at the transcriptional level
by luxS and smcR gene mutation. The results from this study show conclusively
that luxS/smcR quorum sensing endows a global change in gene expression to
V. vulnificus.
en
dc.description.sponsorshipThis study was supported by KOSF, Brain Korea 21, KRF
(KRF-2006-005-J02901) and the Research Institute for Veterinary
Science, Seoul National University, Korea.
en
dc.language.isoen-
dc.publisherWiley-Blackwellen
dc.subjectVibrio vulnificusen
dc.subject2D-PAGEen
dc.subjectDDRT-PCRen
dc.subjectregulonsen
dc.titleIdentification of quorum sensing-related regulons in Vibrio vulnificus by two-dimensional gel electrophoresis and differentially displayed reverse transcriptase PCRen
dc.typeArticleen
dc.contributor.AlternativeAuthor신나리-
dc.contributor.AlternativeAuthor이덕용-
dc.contributor.AlternativeAuthor유한상-
dc.identifier.doi10.1111/j.1574-695X.2007.00236.x-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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