Publications
Detailed Information
Identification of quorum sensing-related regulons in Vibrio vulnificus by two-dimensional gel electrophoresis and differentially displayed reverse transcriptase PCR
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Na-Ri | - |
dc.contributor.author | Lee, Deog Yong | - |
dc.contributor.author | Yoo, Han Sang | - |
dc.date.accessioned | 2009-08-21T08:23:48Z | - |
dc.date.available | 2009-08-21T08:23:48Z | - |
dc.date.issued | 2007-05-14 | - |
dc.identifier.citation | FEMS Immunol Med Microbiol 50: 94-103 | en |
dc.identifier.issn | 0928-8244 | - |
dc.identifier.uri | https://hdl.handle.net/10371/7474 | - |
dc.description.abstract | Vibrio vulnificus is thought to employ a quorum-sensing system to control the
expression of a global gene. In this study, proteomes and transcriptomes of a lacZ null mutant, VvSRDZ, and a luxS–smcR double mutant, VvSRDZSR, were compared with the parent strain, VvAR, by means of two-dimensional gel electrophoresis (2D-PAGE) and differentially displayed reverse transcriptase PCR (DDRT-PCR). 2D-PAGE analysis showed that 36 protein spots were differentially expressed, 14 of which have been identified by peptide-mass fingerprinting. The expression of eight cellular proteins was repressed by luxS and smcR mutation: Zn-dependent protease, 6-phosophofructokinase, periplasmic ABC-type Fe31 transport system, deoxyribose-phosphate aldolase, phosphomannomutase, orotidine-50-phosphate decarboxylase, uridylate kinase, and an unidentified protein. These proteins are involved in virulence, adaptation to environmental stress, biosynthesis of LPS, and cell multiplication. Phage shock protein A, a chemotaxis signal transduction protein, and an uncharacterized low-complexity protein were activated in the cellular components of the luxS-smcR mutant. However, only three proteins, of unknown function, were identified in the extracellular components of the mutants. Analysis of transcriptomes with DDRTPCR showed that two genes, phosphoribosylformylglycinamidine synthase and ATP-dependent protease HslVU protease were regulated at the transcriptional level by luxS and smcR gene mutation. The results from this study show conclusively that luxS/smcR quorum sensing endows a global change in gene expression to V. vulnificus. | en |
dc.description.sponsorship | This study was supported by KOSF, Brain Korea 21, KRF
(KRF-2006-005-J02901) and the Research Institute for Veterinary Science, Seoul National University, Korea. | en |
dc.language.iso | en | - |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Vibrio vulnificus | en |
dc.subject | 2D-PAGE | en |
dc.subject | DDRT-PCR | en |
dc.subject | regulons | en |
dc.title | Identification of quorum sensing-related regulons in Vibrio vulnificus by two-dimensional gel electrophoresis and differentially displayed reverse transcriptase PCR | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 신나리 | - |
dc.contributor.AlternativeAuthor | 이덕용 | - |
dc.contributor.AlternativeAuthor | 유한상 | - |
dc.identifier.doi | 10.1111/j.1574-695X.2007.00236.x | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.