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Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Sung Jae | - |
dc.contributor.author | Shin, Seung Won | - |
dc.contributor.author | Kang, Mi Lan | - |
dc.contributor.author | Lee, Deog Yong | - |
dc.contributor.author | Yang, Moon-Sik | - |
dc.contributor.author | Jang, Yong-Suk | - |
dc.contributor.author | Yoo, Han Sang | - |
dc.date.accessioned | 2009-08-21T08:26:16Z | - |
dc.date.available | 2009-08-21T08:26:16Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | J Vet Sci 2007, 8, 383-392 | en |
dc.identifier.issn | 1229-845X | - |
dc.identifier.uri | http://www.vetsci.org/2007/abstract/383a.html | - |
dc.identifier.uri | https://hdl.handle.net/10371/7476 | - |
dc.description.abstract | We previously induced protective immune response by
oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of the rApxIA antigen derived from the yeast was confirmed by a high survival rate and an ApxIA-specific IgG antibody response (p < 0.01). The highest systemic (IgG) and local (IgA) humoral immune responses to ApxIA and ApxIIA were detected in group E after the third immunization (p < 0.05). The levels of IL-1β and IL-6 after challenge with an A. pleuropneumoniae field isolate did not change significantly in the vaccinated groups. The level of TNF-α increased in a time-dependent manner in group E but was not significantly different after the challenge. After the challenge, the mice in group E had a significantly lower infectious burden and a higher level of protection than the mice in the other groups (p < 0.05). The survival rate in each group was closely correlated to the immune response and histopathological observations in the lung following the challenge. These results suggested that immunity to the ApxIA antigen is required for optimal protection. | en |
dc.description.sponsorship | This study was supported by BioGreen 21 (200503013
4414), RDA, Brain Korea 21, and the Research Institute for Veterinary Sciences, Seoul National University, Korea. | en |
dc.language.iso | en | - |
dc.publisher | 대한수의학회 = The Korean Society of Veterinary Science | en |
dc.subject | Actinobacillus pleuropneumoniae | en |
dc.subject | Apx toxins | en |
dc.subject | oral immunization | en |
dc.subject | protective immunity | en |
dc.title | Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 신성재 | - |
dc.contributor.AlternativeAuthor | 신승원 | - |
dc.contributor.AlternativeAuthor | 강미란 | - |
dc.contributor.AlternativeAuthor | 이덕용 | - |
dc.contributor.AlternativeAuthor | 양문식 | - |
dc.contributor.AlternativeAuthor | 장용석 | - |
dc.contributor.AlternativeAuthor | 유한상 | - |
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