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Cloning, expression and bioassay of canine CTLA4Ig
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Il-Seob | - |
dc.contributor.author | Choi, Eun-Wha | - |
dc.contributor.author | Chung, Jin-Young | - |
dc.contributor.author | Hwang, Cheol Yong | - |
dc.contributor.author | Lee, Chang Woo | - |
dc.contributor.author | Youn, Hwa Young | - |
dc.date.accessioned | 2009-08-24 | - |
dc.date.available | 2009-08-24 | - |
dc.date.issued | 2007-04-09 | - |
dc.identifier.citation | Vet. Immunol. Immunopathol. 118, 12-18 | en |
dc.identifier.issn | 0165-2427 | - |
dc.identifier.uri | https://hdl.handle.net/10371/7521 | - |
dc.description.abstract | Blockade of the B7:CD28 costimulatory pathway has been shown to inhibit humoral immunity, graft rejection, graft versus host disease and ameliorate autoimmune diseases. A soluble chimeric fusion protein, CTLA4Ig, binds to B7 with greater affinity than CD28 and blocks the binding of CD28 to B7. We describe the cloning and expression of canine CTLA4Ig, a recombinant chimeric fusion protein composed of the extracellular domain of canine CTLA-4 and the CH2–CH3 domains of canine immunoglobulin alpha constant region (IGHA) genes, linked via an immunologically inert flexible peptide. The recombinant CTLA4Ig protein of approximately 45 kDa molecular weight was expressed mainly as insoluble inclusion bodies in Escherichia coli. The protein was solubilized in denaturing buffer and purified using nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography followed by refolding. The yield was about 6 mg of recombinant CTLA4Ig per liter of culture. The purified protein was biologically active in one-way mixed lymphocyte reactions, demonstrating immunosuppressive activities in a dose-dependent manner. The findings suggest that recombinant canine CTLA4Ig protein could be valuable in assessing the function of CTLA-4 in the canine immune system and may be effective in autoimmune disease therapy. | en |
dc.description.sponsorship | This work was supported by a Korean Research Foundation Grant (KRF-2006-005-J02902). Further supports were also provided by the Research Institute of Veterinary Science, College of Veterinary Medicine, Seoul National University and the Brain Korea 21 Program for Veterinary Science. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | CTLA-4 | en |
dc.subject | Costimulatory pathway | en |
dc.subject | Chimeric fusion protein | en |
dc.subject | Protein expression | en |
dc.subject | Dog | en |
dc.subject | Mixed lymphocyte reaction | en |
dc.title | Cloning, expression and bioassay of canine CTLA4Ig | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 신일섭 | - |
dc.contributor.AlternativeAuthor | 최은화 | - |
dc.contributor.AlternativeAuthor | 정진영 | - |
dc.contributor.AlternativeAuthor | 황철용 | - |
dc.contributor.AlternativeAuthor | 이창우 | - |
dc.contributor.AlternativeAuthor | 윤화영 | - |
dc.identifier.doi | 10.1016/j.vetimm.2007.03.013 | - |
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