Publications
Detailed Information
IRBIT coordinates epithelial fluid and HCO(3)(-)secretion by stimulating the transporters pNBC1 and CFTR in the murine pancreatic duct
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Dongki | - |
dc.contributor.author | Shcheynikov, Nikolay | - |
dc.contributor.author | Zeng, Weizhong | - |
dc.contributor.author | Ohana, Ehud | - |
dc.contributor.author | Ando, Hideaki | - |
dc.contributor.author | Mikoshiba, Katsuhiko | - |
dc.contributor.author | Muallem, Shmuel | - |
dc.contributor.author | Mizutani, Akihiro | - |
dc.contributor.author | So, Insuk | - |
dc.date.accessioned | 2012-05-22T07:47:32Z | - |
dc.date.available | 2012-05-22T07:47:32Z | - |
dc.date.issued | 2009-01 | - |
dc.identifier.citation | JOURNAL OF CLINICAL INVESTIGATION; Vol.119 1; 193-202 | ko_KR |
dc.identifier.issn | 0021-9738 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76251 | - |
dc.description.abstract | Fluid and HCO(3)(-) secretion are vital functions of secretory epithelia. In most epithelia, this entails HCO(3)(-)entry at the basolateral membrane, mediated by the Na(+)-HCO(3)(-) cotransporter, pNBC1, and exit at the luminal membrane, mediated by a CFTR-SLC26 transporters complex. Here we report that the protein IRBIT (inositol-1,4,5-trisphosphate [IP(3)] receptors binding protein released with IP(3)), a previously identified activator of pNBC1, activates both the basolateral pNBC1 and the luminal CFTR to coordinate fluid and HCO(3)(-) secretion by the pancreatic duct. We used video microscopy and ion selective microelectrodes to measure fluid secretion and Cl(-) and HCO(3)(-) concentrations in cultured murine sealed intralobular pancreatic ducts. Short interference RNA-mediated knockdown of IRBIT markedly inhibited ductal pNBC1 and CFTR activities, luminal Cl- absorption and HCO(3)(-) secretion, and the associated fluid secretion. Single-channel measurements suggested that IRBIT regulated CFTR by reducing channel mean close time. Furthermore, expression of IRBIT constructs in HEK cells revealed that activation of pNBC1 required only the IRBIT PEST domain, while activation of CFTR required multiple IRBIT domains, suggesting that IRBIT activates these transporters by different mechanisms. These findings define IRBIT as a key coordinator of epithelial fluid and HCO(3)(-) secretion and may have implications to all CFTR-expressing epithelia and to cystic fibrosis. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | AMER SOC CLINICAL INVESTIGATION INC | ko_KR |
dc.title | IRBIT coordinates epithelial fluid and HCO(3)(-)secretion by stimulating the transporters pNBC1 and CFTR in the murine pancreatic duct | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 양동기 | - |
dc.contributor.AlternativeAuthor | 소인석 | - |
dc.identifier.doi | 10.1172/JCI36983 | - |
dc.citation.journaltitle | JOURNAL OF CLINICAL INVESTIGATION | - |
dc.description.citedreference | Knauf F, 2001, P NATL ACAD SCI USA, V98, P9425 | - |
dc.description.citedreference | Xie QH, 2002, AM J PHYSIOL-RENAL, V283, pF826, DOI 10.1152/ajprenal.00079.2002 | - |
dc.description.citedreference | Shcheynikov N, 2008, J PHYSIOL-LONDON, V586, P3813, DOI 10.1113/jphysiol.2008.154468 | - |
dc.description.citedreference | Choi JY, 2001, NATURE, V410, P94 | - |
dc.description.citedreference | Wang SS, 2000, CELL, V103, P169 | - |
dc.description.citedreference | Lee MG, 2000, J CLIN INVEST, V105, P1651 | - |
dc.description.citedreference | Baron JH, 2000, MT SINAI J MED, V67, P68 | - |
dc.description.citedreference | Melvin JE, 1999, J BIOL CHEM, V274, P22855 | - |
dc.description.citedreference | Lee MG, 1999, J BIOL CHEM, V274, P14670 | - |
dc.description.citedreference | Ishiguro H, 1998, J PHYSIOL-LONDON, V511, P407 | - |
dc.description.citedreference | Abuladze N, 1998, J BIOL CHEM, V273, P17689 | - |
dc.description.citedreference | Ko SBH, 2002, EMBO J, V21, P5662 | - |
dc.description.citedreference | Ma TH, 2002, J CLIN INVEST, V110, P1651, DOI 10.1172/JCI200216112 | - |
dc.description.citedreference | Ando H, 2003, J BIOL CHEM, V278, P10602 | - |
dc.description.citedreference | GUGGINO WB, 2004, P AM THORAC SOC, V1, P28 | - |
dc.description.citedreference | Ko SBH, 2004, NAT CELL BIOL, V6, P343, DOI 10.1038/ncb1115 | - |
dc.description.citedreference | Fernandez-Salazar MP, 2004, J PHYSIOL-LONDON, V556, P415, DOI 10.1113/jphysiol.2004.061762 | - |
dc.description.citedreference | Steward MC, 2005, ANNU REV PHYSIOL, V67, P377, DOI 10.1146/annurev.physiol.67.031103.153247 | - |
dc.description.citedreference | Melvin JE, 2005, ANNU REV PHYSIOL, V67, P445, DOI 10.1146/annurev.physiol.67.041703.084745 | - |
dc.description.citedreference | SHCHEYNIKOV N, 2006, NOVART FDN SYMP, V273, P177 | - |
dc.description.citedreference | SHCHEYNIKOV N, 2006, NOVART FDN SYMP, V273, P186 | - |
dc.description.citedreference | Devogelaere B, 2006, BIOCHEM BIOPH RES CO, V343, P49, DOI 10.1016/j.bbrc.2006.02.119 | - |
dc.description.citedreference | Shcheynikov N, 2006, J GEN PHYSIOL, V127, P511, DOI 10.1085/jgp.200509392 | - |
dc.description.citedreference | Shirakabe K, 2006, P NATL ACAD SCI USA, V103, P9542, DOI 10.1073/pnas.0602250103 | - |
dc.description.citedreference | Ando H, 2006, MOL CELL, V22, P795, DOI 10.1016/j.molcel.2006.05.017 | - |
dc.description.citedreference | Wang YX, 2006, EMBO J, V25, P5049, DOI 10.1038/sj.emboj.7601387 | - |
dc.description.citedreference | Devogelaere B, 2007, BIOCHEM J, V407, P303, DOI 10.1042/BJ20070361 | - |
dc.description.citedreference | Dorwart MR, 2008, PHYSIOLOGY, V23, P104, DOI 10.1152/physiol.00037.2007 | - |
dc.description.citedreference | Hanrahan JW, 1998, METHOD ENZYMOL, V293, P169 | - |
dc.description.citedreference | Linsdell P, 1997, J GEN PHYSIOL, V110, P355 | - |
dc.description.citedreference | Lee MG, 1997, J BIOL CHEM, V272, P15765 | - |
dc.description.citedreference | Yule DI, 1997, J BIOL CHEM, V272, P9093 | - |
dc.description.citedreference | Sohma Y, 1996, J MEMBRANE BIOL, V154, P53 | - |
dc.description.citedreference | Ishiguro H, 1996, J PHYSIOL-LONDON, V495, P169 | - |
dc.description.citedreference | SASAKI T, 1994, AM J PHYSIOL, V267, pL152 | - |
dc.description.citedreference | ZHAO H, 1994, J GEN PHYSIOL, V104, P57 | - |
dc.description.citedreference | POULSEN JH, 1994, P NATL ACAD SCI USA, V91, P5340 | - |
dc.description.citedreference | PAULAIS M, 1992, J BIOL CHEM, V267, P21558 | - |
dc.description.citedreference | ASHTON N, 1991, J PHYSIOL-LONDON, V435, P533 | - |
dc.description.citedreference | ASHTON N, 1990, J PHYSIOL-LONDON, V427, P471 | - |
dc.description.citedreference | DURIE PR, 1989, ACTA PAEDIATR SC S, V363, P41 | - |
dc.description.tc | 18 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.