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5,7-dihydroxy-3,4,6-trimethoxyflavone inhibits the inflammatory effects induced by Bacteroides fragilis enterotoxin via dissociating the complex of heat shock protein 90 and IκBα and IκB kinase-γ in intestinal epithelial cell culture

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dc.contributor.authorKim, J. M.-
dc.contributor.authorLee, D. H.-
dc.contributor.authorKim, J. S.-
dc.contributor.authorLee, J. Y.-
dc.contributor.authorKim, Y. -J.-
dc.contributor.authorJung, H. C.-
dc.contributor.authorKim, S. I.-
dc.contributor.authorOh, Y. -K.-
dc.contributor.authorPark, H. -G.-
dc.date.accessioned2012-05-23T08:52:41Z-
dc.date.available2012-05-23T08:52:41Z-
dc.date.issued2009-03-
dc.identifier.citationCLINICAL AND EXPERIMENTAL IMMUNOLOGY; Vol.155 3; 541-551ko_KR
dc.identifier.issn0009-9104-
dc.identifier.urihttps://hdl.handle.net/10371/76354-
dc.description.abstractEnterotoxin produced by enterotoxigenic Bacteroides fragilis (BFT) has been associated with mucosal inflammation and diarrhoeal diseases. In this study, the anti-inflammatory molecular mechanism of 5,7-dihydroxy-3,4,6-trimethoxyflavone (eupatilin) was characterized in an HT-29 intestinal epithelial cell line stimulated with BFT. Pre-treatment of HT-29 cells with eupatilin decreased the production significantly of both interleukin (IL)-8 and prostaglandin E(2) induced by BFT in a dose-dependent manner. BFT-activated nuclear factor-kappaB (NF-kappa B) signals in HT-29 cells and pretreatment with eupatilin suppressed NF-kappa B activation that resulted in the significant inhibition of IL-8 and cyclo-oxygenase-2 expression. BFT-induced phosphorylation of both I kappa B alpha and I kappa B kinase (IKK) signals was prevented in eupatilin-pretreated HT-29 cells. Transfection of siRNA for IKK-alpha and IKK-beta decreased the production of IL-8 and prostaglandin E(2); however, the transfection of IKK-beta siRNA showed a more significant reduction of BFT-induced I kappa B alpha phosphorylation compared with that of IKK-alpha siRNA. In addition, herbimycin A, a specific inhibitor of heat shock protein 90 (Hsp90), decreased the BFT-induced activation of IKK and NF-kappa B, suggesting that Hsp90 is associated with a pathway of IKK-NF-kappa B-IL-8/cyclo-oxygenase-2 gene signalling. Furthermore, eupatilin dissociated the complex between Hsp90 and IKK-gamma in BFT-stimulated HT-29 cells. These results suggest that eupatilin can suppress the NF-kappa B signalling pathway by targeting the Hsp90-IKK-gamma complex in intestinal epithelial cells and may attenuate BFT-induced inflammatory responses.ko_KR
dc.language.isoenko_KR
dc.publisherWILEY-BLACKWELLko_KR
dc.subjectBacteroides fragilis enterotoxinko_KR
dc.subjectNF-κBko_KR
dc.subjecteupatilinko_KR
dc.subjectIκB kinase,ko_KR
dc.subjectHsp90ko_KR
dc.title5,7-dihydroxy-3,4,6-trimethoxyflavone inhibits the inflammatory effects induced by Bacteroides fragilis enterotoxin via dissociating the complex of heat shock protein 90 and IκBα and IκB kinase-γ in intestinal epithelial cell cultureko_KR
dc.typeArticleko_KR
dc.identifier.doi10.1111/j.1365-2249.2008.03849.x-
dc.citation.journaltitleCLINICAL AND EXPERIMENTAL IMMUNOLOGY-
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dc.description.tc6-
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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