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The Effect of CpG-Oligodeoxynucleotides with Different Backbone Structures and 3 ` Hexameric Deoxyriboguanosine Run Conjugation on the Treatment of Asthma in Mice

Cited 2 time in Web of Science Cited 1 time in Scopus
Authors
Chang, Yoon-Seok; Kim, Yoon-Keun; Kwon, Hyouk-Soo; Park, Heung-Woo; Kim, You-Young; Cho, Sang-Heon; Min, Kyung-Up
Issue Date
2009-10
Publisher
KOREAN ACAD MEDICAL SCIENCES
Citation
JOURNAL OF KOREAN MEDICAL SCIENCE; Vol.24 5; 860-866
Keywords
AsthmaPoly GCpG-ODNModelsAllergyImmunotherapyAnimal
Abstract
CpG-Oligodeoxynucleotide (ODN) has two backbones. Phosphorothioate backbone (PS) shows a strong immunostimulating effect while phosphodiester (PE) shows little in vivo. 3` hexameric deoxyriboguanosine-run (3` dG(6)-run) conjugation to PE CpG-ODN has been reported to enhance immunostimulation and to protect against asthma when injected at the time of sensitization in mice. We evaluated the treatment effects of PE and PS CpG-ODN with or without 3` dG(6)-run on asthma in pre-sensitized mice. BALB/c mice sensitized with ovalbumin and alum were challenged with 1% ovalbumin on three days. CpG-ODNs (100 mu g) or PBS were injected 4 times; 27 hr before challenge and 3 hr before each challenge (CpG-dG(6:) CpG-ODN with 3` dG(6)-run, PE*-CpG-dG(6): PE-CpG-dG(6) with two PS backbones at the 5` terminus). PE-CpG showed no treatment effect. PE-CpG-dG(6) only increased ovalbumin-specific IgG2a. PE*-CpG-dG(6) increased ovalbumin-specific IgG2a but also reduced BAL fluid eosinophils and airway hyperresponsiveness. PS-CpG increased ovalbumin-specific IgG2a, reduced airway inflammation and airway hyperresponsiveness. PS-CpG-dG(6) was less effective than PS-CpG on airway inflammation and airway hyperresponsiveness. In pre-sensitized mice, PE-CpG required not only 3` dG(6)-run but also the modification of two PS linkages at 5` terminus to inhibit features of asthma. PS-CpG was strong enough to inhibit asthma but PS-CpG-dG(6) was less effective.
ISSN
1011-8934
Language
English
URI
http://hdl.handle.net/10371/76437
DOI
https://doi.org/10.3346/jkms.2009.24.5.860
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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