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Novel Embryoid Body-Based Method to Derive Mesenchymal Stem Cells from Human Embryonic Stem Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Eun Ju | - |
dc.contributor.author | Lee, Ha-Neul | - |
dc.contributor.author | Kang, Hyun-Jae | - |
dc.contributor.author | Kim, Keum-Hyun | - |
dc.contributor.author | Cho, Hyun-Jai | - |
dc.contributor.author | Chung, Hyung-Min | - |
dc.contributor.author | Cho, Mee-Young | - |
dc.contributor.author | Moon, Shin-Yong | - |
dc.contributor.author | Kim, Hyo-Soo | - |
dc.contributor.author | Park, Young-Bae | - |
dc.contributor.author | Oh, Sun-Kyung | - |
dc.contributor.author | Cho, Jaejin | - |
dc.contributor.author | Lee, Jaewon | - |
dc.contributor.author | Hur, Jin | - |
dc.date.accessioned | 2012-05-29T06:10:26Z | - |
dc.date.available | 2012-05-29T06:10:26Z | - |
dc.date.issued | 2010-02 | - |
dc.identifier.citation | TISSUE ENGINEERING PART A; Vol.16 2; 705-715 | ko_KR |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76546 | - |
dc.description.abstract | Application of human embryonic stem cells (hESCs) to stem-cell therapy is not feasible because of the risk of tumorigenicity and rejection. In contrast, human mesenchymal stem cells (hMSCs) are free from the risk of tumorigenicity and also have immune privilege. However, hMSCs obtained from adults have infinite variety in terms of the biological characteristics and functionality. We report here a new derivation method of hMSCs from hESCs. The derivation of hMSCs from three different hESC lines (SNUhES3, CHA3-hESC, and H9) was performed by embryoid bodies formation and subsequent culture with stage-different media without using inductive xenogenic feeder and mechanical selection procedure. The derived cells were morphologically similar to the unique fingerprint-like pattern of hMSCs and grew stably for at least 35 passages in vitro. These cells had hMSCs-like immunophenotypes: negative for CD34 and CD45; positive for CD29, CD44, CD73, CD90, and CD105. They could be differentiated into multiple lineages including osteocytes, chondrocytes, adipocytes, and myocytes. They maintained normal karyotype during the long-term cultivation and did not show tumorigenicity when transplanted into the immunodeficient mice. In conclusion, the new embryoid body-based derivation method of hMSCs from hESCs is simple, safe, and reproducible in three different hESC lines. We expect that this method will provide a more effective and powerful tool to derive hMSCs from various hESC lines. | ko_KR |
dc.description.sponsorship | This study was supported by a grant for Stem Cell Research
Center (SC4210). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | MARY ANN LIEBERT INC | ko_KR |
dc.title | Novel Embryoid Body-Based Method to Derive Mesenchymal Stem Cells from Human Embryonic Stem Cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이은주 | - |
dc.contributor.AlternativeAuthor | 이하늘 | - |
dc.contributor.AlternativeAuthor | 강현재 | - |
dc.contributor.AlternativeAuthor | 김금현 | - |
dc.contributor.AlternativeAuthor | 허진 | - |
dc.contributor.AlternativeAuthor | 조현재 | - |
dc.contributor.AlternativeAuthor | 이재원 | - |
dc.contributor.AlternativeAuthor | 정형민 | - |
dc.contributor.AlternativeAuthor | 조재진 | - |
dc.contributor.AlternativeAuthor | 조미영 | - |
dc.contributor.AlternativeAuthor | 오선경 | - |
dc.contributor.AlternativeAuthor | 문신용 | - |
dc.contributor.AlternativeAuthor | 박영배 | - |
dc.contributor.AlternativeAuthor | 김효수 | - |
dc.identifier.doi | 10.1089/ten.tea.2008.0596 | - |
dc.citation.journaltitle | TISSUE ENGINEERING PART A | - |
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dc.description.tc | 5 | - |
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