Publications

Detailed Information

Gq protein mediates UVB-induced cyclooxygenase-2 expression by stimulating HB-EGF secretion from HaCaT human keratinocytes

DC Field Value Language
dc.contributor.authorSeo, MiRan-
dc.contributor.authorJuhnn, Yong-Sung-
dc.date.accessioned2012-06-05T05:49:58Z-
dc.date.available2012-06-05T05:49:58Z-
dc.date.issued2010-03-05-
dc.identifier.citationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS; Vol.393 2; 190-195ko_KR
dc.identifier.issn0006-291X-
dc.identifier.urihttps://hdl.handle.net/10371/76850-
dc.description.abstractUltraviolet (UV) radiation induces cyclooxygenase-2 expression to produce cellular responses including aging and carcinogenesis in skin. We hypothesised that heterotrimeric G proteins mediate UV-induced COX-2 expression by stimulating secretion of soluble HB-EGF (sHB-EGF). In this study, we aimed to elucidate the role and underlying mechanism of the a subunit of Gq protein (G alpha q) in UVB-induced HB-EGF secretion and COX-2 induction. We found that expression of constitutively active G alpha q (G alpha qQL) augmented UVB-induced HB-EGF secretion. which was abolished by knockdown of G alpha q with shRNA in HaCaT human keratinocytes. G alpha q was found to mediate the UVB-induced HB-EGF secretion by sequential activation of phospholipase C (PLC), protein kinase C delta (PKC delta), and matrix metaloprotease-2 (MMP-2). Moreover, G alpha qQL mediated UVB-induced COX-2 expression in an HH-EGF-, EGFR-, and p38-dependent manner. From these results, we concluded that G alpha q mediates UV-induced COX-2 expression through activation of EGFR by HB-EGF, of which ectodomain shedding was stimulated through sequential activation of PLC, PKC delta and MMP-2 in HaCaT cells. (C) 2010 Elsevier Inc. All rights reserved.ko_KR
dc.language.isoenko_KR
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEko_KR
dc.subjectGαqko_KR
dc.subjectKeratinocytesko_KR
dc.subjectUVBko_KR
dc.subjectHB-EGFko_KR
dc.subjectCOX-2ko_KR
dc.titleGq protein mediates UVB-induced cyclooxygenase-2 expression by stimulating HB-EGF secretion from HaCaT human keratinocytesko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor서미란-
dc.contributor.AlternativeAuthor전용성-
dc.identifier.doi10.1016/j.bbrc.2010.01.085-
dc.citation.journaltitleBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.description.citedreferenceBeltran AE, 2009, J HYPERTENS, V27, P142, DOI 10.1097/HJH.0b013e328317a730-
dc.description.citedreferenceDong KK, 2008, EXP DERMATOL, V17, P1037, DOI 10.1111/j.1600-0625.2008.00747.x-
dc.description.citedreferenceHsieh HL, 2008, BBA-MOL CELL RES, V1783, P1563, DOI 10.1016/j.bbamcr.2008.03.016-
dc.description.citedreferenceKim Y, 2008, J BIOL CHEM, V283, P22513, DOI 10.1074/jbc.M708319200-
dc.description.citedreferencePham H, 2008, BBA-GENE REGUL MECH, V1779, P408, DOI 10.1016/j.bbagrm.2008.05.004-
dc.description.citedreferenceRundhaug JE, 2008, PHOTOCHEM PHOTOBIOL, V84, P322, DOI 10.1111/j.1751-1097.2007.00261.x-
dc.description.citedreferenceKim SY, 2008, J BIOL CHEM, V283, P1350, DOI 10.1074/jbc.M702344200-
dc.description.citedreferenceHuang W, 2008, DIGEST DIS SCI, V53, P163, DOI 10.1007/s10620-007-9838-9-
dc.description.citedreferenceSeo MR, 2007, J BIOL CHEM, V282, P24720-
dc.description.citedreferenceZhou WL, 2007, AM J PHYSIOL-HEART C, V292, pH2773, DOI 10.1152/ajpheart.01018.2006-
dc.description.citedreferenceChun KS, 2007, CANCER RES, V67, P2015, DOI 10.1158/0008-5472.CAN-06-3617-
dc.description.citedreferenceNagase H, 2006, CARDIOVASC RES, V69, P562, DOI 10.1016/j.cardiores.2005.12.002-
dc.description.citedreferencePakozdi A, 2006, ARTHRITIS RES THER, V8, DOI 10.1186/ar2021-
dc.description.citedreferenceHusain S, 2005, INVEST OPHTH VIS SCI, V46, P1706, DOI 10.1167/iovs.04-0993-
dc.description.citedreferenceLucchesi PA, 2004, CIRCULATION, V110, P3587, DOI 10.1161/01.CIR.0000148780.36121.47-
dc.description.citedreferenceWoo CH, 2004, J IMMUNOL, V173, P6973-
dc.description.citedreferenceDeo DD, 2004, J BIOL CHEM, V279, P3497, DOI 10.1074/jbc.M304497200-
dc.description.citedreferenceLe Gall SM, 2003, J BIOL CHEM, V278, P45255, DOI 10.1074/jbc.M307745200-
dc.description.citedreferenceZaczynska E, 2003, INFLAMMATION, V27, P307-
dc.description.citedreferenceTsai CH, 2003, ORAL SURG ORAL MED O, V95, P710, DOI 10.1067/moe.2003.121-
dc.description.citedreferenceTakenobu H, 2003, J BIOL CHEM, V278, P17255, DOI 10.1074/jbc.M211835200-
dc.description.citedreferenceSlice LW, 2003, J CELL PHYSIOL, V194, P127, DOI 10.1002/jcp.10195-
dc.description.citedreferenceBachelor MA, 2002, ONCOGENE, V21, P7092, DOI 10.1038/sj.onc.1205855-
dc.description.citedreferenceFukunaga M, 2001, BIOCHEM BIOPH RES CO, V289, P573-
dc.description.citedreferenceJOHN A, 2001, PATHOL ONCOL RES, V7, P14-
dc.description.citedreferenceAbbott KL, 2000, J MOL CELL CARDIOL, V32, P391-
dc.description.citedreferenceSmith WL, 2000, ANNU REV BIOCHEM, V69, P145-
dc.description.citedreferenceFu SL, 1999, P NATL ACAD SCI USA, V96, P5716-
dc.description.citedreferenceClapham DE, 1997, ANNU REV PHARMACOL, V37, P167-
dc.description.citedreferenceKOIVUKANGAS V, 1994, ACTA DERM-VENEREOL, V74, P279-
dc.description.citedreferenceMARIKOVSKY M, 1993, P NATL ACAD SCI USA, V90, P3889-
dc.description.citedreferenceKRAMER M, 1993, J BIOL CHEM, V268, P6734-
dc.description.citedreferenceLEE CH, 1992, J BIOL CHEM, V267, P16044-
dc.description.citedreferenceGILMAN AG, 1987, ANNU REV BIOCHEM, V56, P615-
dc.description.tc2-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share