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Cell-free production of functional antibody fragments
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, In-Seok | - |
dc.contributor.author | Lee, Ji-Chul | - |
dc.contributor.author | Lee, Myung-shin | - |
dc.contributor.author | Chung, Jun-ho | - |
dc.contributor.author | Kim, Dong-Myung | - |
dc.date.accessioned | 2012-06-05T05:57:16Z | - |
dc.date.available | 2012-06-05T05:57:16Z | - |
dc.date.issued | 2010-01 | - |
dc.identifier.citation | BIOPROCESS AND BIOSYSTEMS ENGINEERING; Vol.33 1; 127-132 | ko_KR |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76852 | - |
dc.description.abstract | Botulinum neurotoxin serotype B (BoNT/B)-specific Fab was expressed in a cell-free protein synthesis system derived from an E. coli extract. The cell-free synthesized antibody fragment was found to be effective in neutralizing the toxicity of BoNT/B in animal studies. Expression of functional Fab required an appropriately controlled and stably maintained redox potential. Under an optimized redox condition, the cell extract, whose disulfide reducing activity had been exhausted, could generate bio-functional Fab molecules. Use of a cell extract enriched with molecular chaperones (GroEL/ES) and disulfide bond isomerases were effective in obtaining larger quantities of functional Fab. Under the optimized reaction conditions, approximately 30 mu g of functional Fab was obtained after purification from 1 mL reaction mixture. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | SPRINGER | ko_KR |
dc.subject | Cell-free protein synthesis system | ko_KR |
dc.subject | Botulinum neurotoxin | ko_KR |
dc.subject | Antigen-binding fragment (Fab) | ko_KR |
dc.subject | Disulfide bond | ko_KR |
dc.subject | Antibody | ko_KR |
dc.title | Cell-free production of functional antibody fragments | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 오인석 | - |
dc.contributor.AlternativeAuthor | 이지철 | - |
dc.contributor.AlternativeAuthor | 이명신 | - |
dc.contributor.AlternativeAuthor | 정준호 | - |
dc.contributor.AlternativeAuthor | 김동명 | - |
dc.identifier.doi | 10.1007/s00449-009-0372-3 | - |
dc.citation.journaltitle | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
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dc.description.tc | 2 | - |
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