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Qualitative and quantitative comparison of N-glycans between pig endothelial and islet cells by high-performance liquid chromatography and mass spectrometry-based strategy
DC Field | Value | Language |
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dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Gil, Geun-Cheol | - |
dc.contributor.author | Jang, Kyung-Soon | - |
dc.contributor.author | Lee, Sukmook | - |
dc.contributor.author | Kim, Jung-Sik | - |
dc.contributor.author | Park, Chung-Gyu | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Harvey, David J. | - |
dc.contributor.author | Chung, Junho | - |
dc.contributor.author | Kim, Hyoung-Il | - |
dc.date.accessioned | 2012-06-11T02:29:59Z | - |
dc.date.available | 2012-06-11T02:29:59Z | - |
dc.date.issued | 2009-07 | - |
dc.identifier.citation | JOURNAL OF MASS SPECTROMETRY; Vol.44 7; 1087-1104 | ko_KR |
dc.identifier.issn | 1076-5174 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76947 | - |
dc.description.abstract | N-glycan structures released from miniature pig endothelial and islet cells were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) negative ion electrospray ionization (ESI) MS/MS and normal-phase high performance liquid chromatography (NP-HPLC) combined with exoglycosidase digestion. Totally, the identified structures were 181 N-glycans including 129 sialylated and 18 alpha-galactosylated glycans from pig endothelial cells and 80 N-glycans including 41 sialylated and one alpha-galactosylated glycans from pig islet cells. The quantity of the alpha-galactosylated glycans from pig islet cells was certainly neglectable compared to pig endothelial cells. A number of NeuGc-terminated N-glycans (80 from pig endothelial cells and 13 from pig islet cells) are newly detected by our mass spectrometric strategies. The detailed structural information will be a matter of great interest in organ or cell xenotransplantation using alpha 1,3-galactosyltransferase gene-knockout (GaIT-KO) pig. Copyright (C) 2009 John Wiley & Sons, Ltd. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | JOHN WILEY & SONS LTD | ko_KR |
dc.subject | xenotransplantation | ko_KR |
dc.subject | pig islet cells | ko_KR |
dc.subject | pig endothelial cell | ko_KR |
dc.subject | N-glycolyl-neuraminic acid | ko_KR |
dc.subject | alpha-galactose | ko_KR |
dc.subject | mass spectrometry | ko_KR |
dc.title | Qualitative and quantitative comparison of N-glycans between pig endothelial and islet cells by high-performance liquid chromatography and mass spectrometry-based strategy | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김윤곤 | - |
dc.contributor.AlternativeAuthor | 길근철 | - |
dc.contributor.AlternativeAuthor | 장경순 | - |
dc.contributor.AlternativeAuthor | 이석묵 | - |
dc.contributor.AlternativeAuthor | 김형일 | - |
dc.contributor.AlternativeAuthor | 김정식 | - |
dc.contributor.AlternativeAuthor | 정준호 | - |
dc.contributor.AlternativeAuthor | 박정규 | - |
dc.contributor.AlternativeAuthor | 김병지 | - |
dc.identifier.doi | 10.1002/jms.1587 | - |
dc.citation.journaltitle | JOURNAL OF MASS SPECTROMETRY | - |
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