Publications
Detailed Information
Stimulatory heterotrimeric G protein augments gamma ray-induced apoptosis by up-regulation of Bak expression via CREB and AP-1 in H1299 human lung cancer cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Yoon Jung | - |
dc.contributor.author | Kim, So-Young | - |
dc.contributor.author | Oh, Jung-Min | - |
dc.contributor.author | Juhnn, Yong-Sung | - |
dc.date.accessioned | 2012-06-28T02:29:46Z | - |
dc.date.available | 2012-06-28T02:29:46Z | - |
dc.date.issued | 2009-08-31 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE; Vol.41; no. 8; 592-600 | ko_KR |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://hdl.handle.net/10371/77733 | - |
dc.description.abstract | Stimulatory heterotrimeric GTP-binding proteins (Gs protein) stimulate cAMP generation in response to various signals, and modulate various cellular phenomena such as proliferation and apoptosis. This study aimed to investigate the effect of Gs proteins on gamma ray-induced apoptosis of lung cancer cells and its molecular mechanism, as an attempt to develop a new strategy to improve the therapeutic efficacy of gamma radiation. Expression of constitutively active mutant of the a subunit of Gs (G alpha sQL) augmented gamma ray-induced apoptosis via mitochondrial dependent pathway when assessed by clonogenic assay, FACS analysis of PI stained cells, and western blot analysis of the cytoplasmic translocation of cytochrome C and the cleavage of caspase-3 and ploy(ADP-ribose) polymerase (PARP) in H1299 human lung cancer cells. GasQL up-regulated the Bak expression at the levels of protein and mRNA. Treatment with inhibitors of PKA (H89), SP600125 (JNK inhibitor), and a CRE-decoy blocked G alpha sQL-stimulated Bak reporter luciferase activity. Expression of G alpha sQL increased basal and gamma ray-induced luciferase activity of cAMP response element binding protein (CREB) and AP-1, and the binding of CREB and AP-1 to Bak promoter. Furthermore, prostaglandin E2, a Gas activating signal, was found to augment gamma ray-induced apoptosis, which was abolished by treatment with a prostanoid receptor antagonist. These results indicate that Gas augments gamma ray-induced apoptosis by up-regulation of Bak expression via CREB and AP-1 in H1299 lung cancer cells, suggesting that the efficacy of radiotherapy of lung cancer may be improved by modulating Gs signaling pathway. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | ko_KR |
dc.subject | apoptosis | ko_KR |
dc.subject | bcl-2 homologous antagonist-killer protein | ko_KR |
dc.subject | gamma rays | ko_KR |
dc.subject | receptors, G-protein-coupled | ko_KR |
dc.subject | heterotrimeric GTP-binding protein | ko_KR |
dc.subject | cyclic AMP | ko_KR |
dc.title | Stimulatory heterotrimeric G protein augments gamma ray-induced apoptosis by up-regulation of Bak expression via CREB and AP-1 in H1299 human lung cancer cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 최윤정 | - |
dc.contributor.AlternativeAuthor | 김소영 | - |
dc.contributor.AlternativeAuthor | 오정민 | - |
dc.contributor.AlternativeAuthor | 전용성 | - |
dc.identifier.doi | 10.3858/emm.2009.41.8.065 | - |
dc.citation.journaltitle | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.description.citedreference | Seo M, 2009, BIOCHEM BIOPH RES CO, V381, P153, DOI 10.1016/j.bbrc.2009.01.188 | - |
dc.description.citedreference | Decker RH, 2008, SEMIN RESP CRIT CARE, V29, P285, DOI 10.1055/s-2008-1076748 | - |
dc.description.citedreference | Taylor RC, 2008, NAT REV MOL CELL BIO, V9, P231, DOI 10.1038/nrm2312 | - |
dc.description.citedreference | Kim SY, 2008, J BIOL CHEM, V283, P1350, DOI 10.1074/jbc.M702344200 | - |
dc.description.citedreference | Youle RJ, 2008, NAT REV MOL CELL BIO, V9, P47, DOI 10.1038/nrm2308 | - |
dc.description.citedreference | Kim SY, 2007, EXP MOL MED, V39, P583 | - |
dc.description.citedreference | Wang W, 2007, CLIN CANCER RES, V13, P4891, DOI 10.1158/1078-0432.CCR-07-0416 | - |
dc.description.citedreference | Siu YT, 2007, FEBS J, V274, P3224, DOI 10.1111/j.1742-4658.2007.05884.x | - |
dc.description.citedreference | Sugimoto Y, 2007, J BIOL CHEM, V282, P11613, DOI 10.1074/jbc.R600038200 | - |
dc.description.citedreference | Adams JM, 2007, ONCOGENE, V26, P1324, DOI 10.1038/sj.onc.1210220 | - |
dc.description.citedreference | POTTI A, 2006, EXPERT OPIN BIOL TH, V6, P709 | - |
dc.description.citedreference | Zhang XM, 2005, P NATL ACAD SCI USA, V102, P4459, DOI 10.1073/pnas.0501076102 | - |
dc.description.citedreference | Yano T, 2003, KIDNEY INT, V64, P2052 | - |
dc.description.citedreference | Lewerenz J, 2003, J NEUROCHEM, V87, P522, DOI 10.1046/j.1471-4159.2003.02019.x | - |
dc.description.citedreference | Chen M, 2002, APOPTOSIS, V7, P313 | - |
dc.description.citedreference | Baliga BC, 2002, HEMATOL ONCOL, V20, P63, DOI 10.1002/hon.685 | - |
dc.description.citedreference | Wei MC, 2001, SCIENCE, V292, P727 | - |
dc.description.citedreference | Lizcano JM, 2000, BIOCHEM J, V349, P547 | - |
dc.description.citedreference | Gu CH, 2000, J BIOL CHEM, V275, P20726 | - |
dc.description.citedreference | Greenlee RT, 2000, CA-CANCER J CLIN, V50, P7 | - |
dc.description.citedreference | Geng YJ, 1999, CIRC RES, V84, P34 | - |
dc.description.citedreference | Daaka Y, 1997, P NATL ACAD SCI USA, V94, P2180 | - |
dc.description.citedreference | CHITTENDEN T, 1995, NATURE, V374, P733 | - |
dc.description.citedreference | GONG JP, 1993, CANCER RES, V53, P5096 | - |
dc.description.citedreference | CLAPHAM DE, 1993, NATURE, V365, P403 | - |
dc.description.citedreference | CONKLIN BR, 1993, CELL, V73, P631 | - |
dc.description.citedreference | BROWN AM, 1991, ADV EXP MED BIOL, V308, P119 | - |
dc.description.citedreference | REID BJ, 1987, GASTROENTEROLOGY, V93, P1 | - |
dc.description.tc | 6 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.