S-Space College of Medicine/School of Medicine (의과대학/대학원) Obstetrics & Gynecology (산부인과전공) Journal Papers (저널논문_산부인과학전공)
BMP2 Induces Direct Differentiation into Cardiomyocytes from Human Embryonic Stem Cells during Short-Tem Culture In Vitro
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- Issue Date
- SAGE PUBLICATIONS INC
- REPRODUCTIVE SCIENCES; Vol.17 3; 241A-241A
- Objective: Human embryonic stem cells (hESCs) can proliferate indefi nitely and
differentiate into most of cell types in vitro. Previous studies of differentiation
into cardiomyocytes from hESCs generally used embryoid body (EB) and
growth factors. However, the effi ciency of reported differentiation strategy
using EB was not suffi cient enough for further clinical application.
Methods: We tried to develop an effi cient protocol using combination of activin
A and bone morphogenetic protein-2 (BMP2) to induce direct differentiation
from hESCs into cardiomyocytes. This was an attempt to develop a novel
effi cient protocol.
Results: Using our differentiation protocol, beating cardiomyocytes were
generated at 14 days and the beating lasted for more than 3 weeks in vitro.
Expression of undifferentiated state marker Oct4 was down-regulated and
mesodermal marker Brachyury was expressed at early stage. Thereafter,
expressions of cardiac-specifi c markers such as Nkx 2.5, cardiac troponin
(cTn) I, myosin heavy chain (MHC), α-actinin and smooth muscle actin were
confi rmed at 20 days. The expression of calcium channels markers such as
Kv 1.4, 2.1, 4.3, HCN1, 2, 4 was detected from early stage of differentiation
Conclusion: We demonstrated that differentiation from hESCs into
cardiomyocytes was successfully induced by direct differentiation protocol
using BMP2 during a short-term culture in vitro (SC1150 and No. 2009-
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