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Differential Roles of Matrix Metalloproteinase-9 and-2, Depending on Proliferation or Differentiation of Retinoblastoma Cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Jeong Hun | - |
dc.contributor.author | Kim, Jin Hyoung | - |
dc.contributor.author | Cho, Chang Sik | - |
dc.contributor.author | Jun, Hyoung Oh | - |
dc.contributor.author | Yu, Young Suk | - |
dc.contributor.author | Kim, Kyu-Won | - |
dc.contributor.author | Kim, Dong Hun | - |
dc.date.accessioned | 2012-06-29T02:20:33Z | - |
dc.date.available | 2012-06-29T02:20:33Z | - |
dc.date.issued | 2010-03 | - |
dc.identifier.citation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE; Vol.51 3; 1783-1788 | ko_KR |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://hdl.handle.net/10371/77889 | - |
dc.description.abstract | PURPOSE. To investigate the differential roles of matrix metalloproteinase (MMP)-9 and MMP-2 in the proliferation or differentiation of retinoblastoma cells. METHODS. Cell proliferation assay with an MMP-9 inhibitor and cell viability assay with an MMP-2 inhibitor were performed in retinoblastoma cells with 5 ng/mL fibroblast growth factor 2 for proliferation, 0.1% bovine serum albumin for differentiation, or reverse transcriptase-polymerase chain reaction (RTPCR) for MMP-9, MMP-2, and their tissue inhibitors TIMP-1 and TIMP-2. Immunohistochemistry for MMP-2 and nm23 was performed using an experimental model of retinoblastoma. With the use of an MMP-2 inhibitor, Western blot analysis was performed for neurofilament, extracellular signal-regulated kinases 1 and 2 (ERK 1/2), and phospho-ERK 1/2, and neurite length was measured in differentiated retinoblastoma cells. RESULTS. With the proliferation of retinoblastoma cells, MMP-9 expression was upregulated without alteration of MMP-2, TIMP-1, or TIMP-2. However, proliferation was not affected by the inhibition of MMP-9 activity. Interestingly, only MMP-2 expression, colocalized with differentiated cells in retinoblastoma tissue, was significantly increased in the differentiation of retinoblastoma cells. Inhibition of MMP-2 activity did not affect cellular viability but attenuated neurite outgrowth and neurofilament expression of differentiated retinoblastoma cells, which was mediated through the suppression of ERK 1/2 activation. CONCLUSIONS. The authors suggest that differential expression of MMP-9 and -2 could reflect biological features, such as proliferation and differentiation, of retinoblastoma cells. In particular, MMP-2 could be directly involved in the regulation of differentiation of retinoblastoma cells. Therefore, therapeutic targeting to MMP-2 may prove useful for reducing malignancy through the differentiation of retinoblastoma cells. (In-vest Ophthalmol Vis Sci. 2010;51:1783-1788) DOI:10.1167/iovs.09-3990 | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | ko_KR |
dc.title | Differential Roles of Matrix Metalloproteinase-9 and-2, Depending on Proliferation or Differentiation of Retinoblastoma Cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김정훈 | - |
dc.contributor.AlternativeAuthor | 김진형 | - |
dc.contributor.AlternativeAuthor | 조창식 | - |
dc.contributor.AlternativeAuthor | 전형오 | - |
dc.contributor.AlternativeAuthor | 김동헌 | - |
dc.contributor.AlternativeAuthor | 유영석 | - |
dc.contributor.AlternativeAuthor | 김규원 | - |
dc.identifier.doi | 10.1167/iovs.09-3990 | - |
dc.citation.journaltitle | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
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dc.description.tc | 2 | - |
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