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Activation of toll-like receptors 2, 3 or 5 induces matrix metalloproteinase-1 and-9 expression with the involvement of MAPKs and NF-κB in human epidermal keratinocytes

Cited 38 time in Web of Science Cited 40 time in Scopus
Authors
Lee, Youngae; Kim, Hyunjung; Kim, Sangmin; Kim, Kyu Han; Chung, Jin Ho
Issue Date
2010-08
Publisher
WILEY-BLACKWELL
Citation
EXPERIMENTAL DERMATOLOGY; Vol.19(8); E44-E49
Keywords
keratinocytestoll-like receptorsmatrix metalloproteinase-1matrix metalloproteinase-9
Abstract
Toll-like receptors (TLRs) on epidermal keratinocytes are the first line of defense against microbe invasion, and matrix metalloproteases (MMPs) regulate inflammation, cell migration and wound healing. In this study, we demonstrate that the mRNA and protein expressions of MMP-1 and MMP-9 in human epidermal keratinocytes are induced by ligands for TLR2, TLR3 and TLR5 [Pam3CSK4, Poly(I:C) and flagellin, respectively] in a dose-dependent manner. We also found that the ligands for TLR2, TLR3 and TLR5 activate the MAP kinases, JNK and p38 MAPK, but not ERK1/2. Furthermore, treatment with the ligands for TLR2, TLR3 and TLR5 also induced the degradation of I kappa B-alpha and activated the nuclear translocation of NF-kappa B. MMP-1 induction by the ligands for TLR2, TLR3 and TLR5 was inhibited by pretreatment with BAY11-7082 (NF-kappa B inhibitor) or SP600125 (JNK inhibitor), whereas MMP-9 expression was inhibited by pretreatment with BAY11-7082, SP600125 or SB203580. These findings demonstrate that the activation of TLR2, TLR3 or TLR5 induces the expression of MMP-1 and MMP-9 in human epidermal keratinocytes. In addition, NF-kappa B or JNK mediated the MMP-1 expression induced by TLR2, TLR3 and TLR5, whereas NF-kappa B, JNK or p38 MAPK mediated the MMP-9 expression induced by TLR2, TLR3 and TLR5.
ISSN
0906-6705
Language
English
URI
https://hdl.handle.net/10371/78337
DOI
https://doi.org/10.1111/j.1600-0625.2009.00963.x
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College of Medicine/School of Medicine (의과대학/대학원)Dermatology (피부과학전공)Journal Papers (저널논문_피부과학전공)
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