Publications
Detailed Information
Potential redox-sensitive Akt activation by dopamine activates Bad and promotes cell death in melanocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Hye-Ryung | - |
dc.contributor.author | Shin, Jung-Won | - |
dc.contributor.author | Lee, Hyun-Kyoung | - |
dc.contributor.author | Kim, Jin-Young | - |
dc.contributor.author | Youn, Sang-Woong | - |
dc.contributor.author | Park, Kyoung Chan | - |
dc.contributor.author | Huh, Chang-Hun | - |
dc.date.accessioned | 2012-07-04T01:31:10Z | - |
dc.date.available | 2012-07-04T01:31:10Z | - |
dc.date.issued | 2010-06 | - |
dc.identifier.citation | OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; Vol.3(3); 219-224 | ko_KR |
dc.identifier.issn | 1942-0900 | - |
dc.identifier.uri | https://hdl.handle.net/10371/78364 | - |
dc.description.abstract | Dopamine (DA) is a well known oxidative neurotoxin. In addition, Akt has been reported to deliver a survival signal that inhibits apoptosis. However, it has also been reported that chronic Akt activation leads to apoptosis in response to oxidative stress. The objective of the present study was to investigate the possible role of the Akt pathway in vitiligo and its possible relationship with DA-induced cell death using Mel-Ab cells. Cultured Mel-Ab cells were treated with DA with and without N-Acetyl-L-cysteine (NAC), which is known to have antioxidative properties. Cell viability was then assessed by a crystal violet assay and Annexin staining was performed. The changes in the expression of Akt were analyzed by western blot analysis. The cell viability was reduced by approximately 60% in response to treatment with 500 mu M DA, and NAC effectively prevented this cytotoxic effect. Likewise, treatment with DA produced numerous Annexin positive cells, while treatment with NAC prevented this apoptotic cell death. Akt was slowly phosphorylated after treatment with DA, while NAC clearly inhibited the DA-induced Akt activation. Western blot analysis also showed that treatment with DA induced the activation of Bad. Finally, LY294002 exerted a protective effect against DA-induced apoptotic cell death. DA may induce redox-sensitive Akt activation and increase the level of Bad, which can promote cell death by heterodimerization with survival proteins. Moreover, NAC effectively protects against DA-induced melanocyte death via inhibition of DA-induced Akt activation. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | LANDES BIOSCIENCE | ko_KR |
dc.subject | Akt | ko_KR |
dc.subject | melanocyte | ko_KR |
dc.subject | dopamine | ko_KR |
dc.subject | N-Acetyl-L-cysteine | ko_KR |
dc.subject | bad | ko_KR |
dc.title | Potential redox-sensitive Akt activation by dopamine activates Bad and promotes cell death in melanocytes | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 최혜령 | - |
dc.contributor.AlternativeAuthor | 신정원 | - |
dc.contributor.AlternativeAuthor | 이현경 | - |
dc.contributor.AlternativeAuthor | 김진영 | - |
dc.contributor.AlternativeAuthor | 허창훈 | - |
dc.contributor.AlternativeAuthor | 윤상웅 | - |
dc.contributor.AlternativeAuthor | 박경찬 | - |
dc.identifier.doi | 10.4161/oxim.3.3.8 | - |
dc.citation.journaltitle | OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | - |
dc.description.citedreference | KIM W, 2010, J RADIAT RE IN PRESS | - |
dc.description.citedreference | Arciuch VGA, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0007523 | - |
dc.description.citedreference | Fisher-Wellman K, 2009, OXID MED CELL LONGEV, V2, P43 | - |
dc.description.citedreference | Lahaie-Collins V, 2008, OXID MED CELL LONGEV, V1, P54 | - |
dc.description.citedreference | DUFOUR C, 2008, AM J PHYSIOL-ENDOC M, V294, P794 | - |
dc.description.citedreference | Park ES, 2007, J DERMATOL SCI, V47, P141, DOI 10.1016/j.jdermsci.2007.03.009 | - |
dc.description.citedreference | van Gorp AGM, 2006, CANCER RES, V66, P10760, DOI 10.1158/0008-5472.CAN-06-1111 | - |
dc.description.citedreference | Lu B, 2006, J IMMUNOL, V176, P6785 | - |
dc.description.citedreference | Cucchi ML, 2003, PIGM CELL RES, V16, P111 | - |
dc.description.citedreference | Pedrosa R, 2002, BRIT J PHARMACOL, V137, P1305, DOI 10.1038/sj.bjp.0704982 | - |
dc.description.citedreference | Cho H, 2001, J BIOL CHEM, V276, P38349, DOI 10.1074/jbc.C100462200 | - |
dc.description.citedreference | Blum D, 2001, PROG NEUROBIOL, V65, P135 | - |
dc.description.citedreference | Cho H, 2001, SCIENCE, V292, P1728 | - |
dc.description.citedreference | Oka M, 2000, J INVEST DERMATOL, V115, P699 | - |
dc.description.citedreference | Cucchi ML, 2000, PIGM CELL RES, V13, P28 | - |
dc.description.citedreference | Masure S, 1999, EUR J BIOCHEM, V265, P353 | - |
dc.description.citedreference | LaVoie MJ, 1999, J NEUROSCI, V19, P1484 | - |
dc.description.citedreference | Coffer PJ, 1998, BIOCHEM J, V335, P1 | - |
dc.description.citedreference | delPeso L, 1997, SCIENCE, V278, P687 | - |
dc.description.citedreference | Ahmed NN, 1997, P NATL ACAD SCI USA, V94, P3627 | - |
dc.description.citedreference | KauffmanZeh A, 1997, NATURE, V385, P544 | - |
dc.description.citedreference | White E, 1996, GENE DEV, V10, P1 | - |
dc.description.citedreference | VERMES I, 1995, J IMMUNOL METHODS, V184, P39 | - |
dc.description.citedreference | YANG E, 1995, CELL, V80, P285 | - |
dc.description.citedreference | DOOLEY TP, 1994, SKIN PHARMACOL, V7, P188 | - |
dc.description.citedreference | MORRONE A, 1992, PIGM CELL RES, V5, P65 | - |
dc.description.tc | 2 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.