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In vivo alternative testing with zebrafish in ecotoxicology
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seok, Seung-Hyeok | - |
dc.contributor.author | Baek, Min-Won | - |
dc.contributor.author | Lee, Hui-Young | - |
dc.contributor.author | Kim, Dong-Jae | - |
dc.contributor.author | Na, Yi-Rang | - |
dc.contributor.author | Noh, Kyoung-Jin | - |
dc.contributor.author | Park, Sung-Hoon | - |
dc.contributor.author | Lee, Hyun-Kyoung | - |
dc.contributor.author | Lee, Byoung-Hee | - |
dc.contributor.author | Park, Jae Hak | - |
dc.date.accessioned | 2009-08-27 | - |
dc.date.available | 2009-08-27 | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | J Vet Sci 2008, 9, 351-357 | en |
dc.identifier.issn | 1229-845X | - |
dc.identifier.uri | http://www.vetsci.org/2008/abstract/351a.html | - |
dc.identifier.uri | https://hdl.handle.net/10371/7899 | - |
dc.description.abstract | Although rodents have previously been used in
ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhREEGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3,4,4,5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo- p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies. | en |
dc.description.sponsorship | We acknowledge financial support from a Korea Research
Foundation Grant (KRF-005-E00077), BK21 Program for Veterinary Science and additional financial support from the Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Korea. | en |
dc.language.iso | en | - |
dc.publisher | 대한수의학회 = The Korean Society of Veterinary Science | en |
dc.subject | aryl hydrocarbon receptor | en |
dc.subject | enhanced green fluorescent | en |
dc.subject | zebrafish | en |
dc.title | In vivo alternative testing with zebrafish in ecotoxicology | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 석승혁 | - |
dc.contributor.AlternativeAuthor | 백민원 | - |
dc.contributor.AlternativeAuthor | 이희영 | - |
dc.contributor.AlternativeAuthor | 김동재 | - |
dc.contributor.AlternativeAuthor | 나이랑 | - |
dc.contributor.AlternativeAuthor | 노경진 | - |
dc.contributor.AlternativeAuthor | 박성훈 | - |
dc.contributor.AlternativeAuthor | 이현경 | - |
dc.contributor.AlternativeAuthor | 이병희 | - |
dc.contributor.AlternativeAuthor | 박재학 | - |
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