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Neutrophil Migration in Opposing Chemoattractant Gradients Using Microfluidic Chemotaxis Devices
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lin, Francis | - |
dc.contributor.author | Nguyen, Connie Minh-Canh | - |
dc.contributor.author | Wang, Shur-Jen | - |
dc.contributor.author | Saadi, Wajeeh | - |
dc.contributor.author | Gross, Steven P. | - |
dc.contributor.author | Jeon, Noo Li | - |
dc.date.accessioned | 2009-08-28T05:09:55Z | - |
dc.date.available | 2009-08-28T05:09:55Z | - |
dc.date.issued | 2005-04 | - |
dc.identifier.citation | Ann. Biomed. Eng. 33:475-482, 2005 | en |
dc.identifier.issn | 0090-6964 (print) | - |
dc.identifier.issn | 1573-9686 (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/7988 | - |
dc.description.abstract | Neutrophils migrating in tissue respond to complex overlapping signals generated by a variety of chemotactic factors (CFs). Previous studies suggested a hierarchy between bacteriaderived CFs and host-derived CFs but could not differentiate neutrophil
response to potentially equal host-derived CFs (IL-8 and LTB4). This paper reports neutrophil migration in conflicting gradients of IL-8 and LTB4 using a microfluidic chemotaxis device that can generate stable and well-defined gradients. We quantitatively characterized the movement of cells from time-lapse images. Neutrophils migrate more efficiently toward single IL-8 gradients than single LTB4 gradients as measured by the effective chemotactic index (ECI). In opposing gradients of IL-8 and LTB4, neutrophils show obvious chemotaxis toward a distant gradient, consistent with previous reports. When an opposing gradient of LTB4 is present, neutrophils show less effective chemotaxis toward IL-8 than when they are in a gradient of IL-8 alone. In contrast, the chemotactic response of neutrophils to LTB4 is not reduced in opposing gradients as compared to that in a single LTB4 gradient. These results indicate that the presence of one host-derived CF modifies the response of neutrophils to a second CF suggesting a subtle hierarchy between them. | en |
dc.description.sponsorship | F. Lin thanks the National Institutes of Health General
Medical Science Grant (GM-66051) for a fellowship. We thank the excellent service provided by the General Clinical Research Center (GCRC) at UC Irvine. This research was supported by NSF (DBI-0138055) and NIH (R03AIO055033). | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | Chemotaxis | en |
dc.subject | Gradient | en |
dc.subject | Hierarchy | en |
dc.subject | Microfluidic | en |
dc.subject | Migration | en |
dc.title | Neutrophil Migration in Opposing Chemoattractant Gradients Using Microfluidic Chemotaxis Devices | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 전누리 | - |
dc.identifier.doi | 10.1007/s10439-005-2503-6 | - |
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