Publications
Detailed Information
Rho-associated kinase (ROCK) inhibition reverses low cell activity on hydrophobic surfaces
Cited 18 time in
Web of Science
Cited 18 time in Scopus
- Authors
- Issue Date
- 2009-08-28
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.386, No.3, pp.499-503
- Keywords
- Hydrophobicity ; Rho-associated kinase ; Y-27632 ; Osteoblast ; Cell cycle ; Migration ; Adhesion ; Tissue engineering ; Biomaterial
- Abstract
- Hydrophobic polymers do not offer an adequate scaffold surface for cells to attach, migrate, proliferate, and differentiate. Thus, hydrophobic scaffolds for tissue engineering have traditionally been physicochemically modified to enhance cellular activity. However, modifying the surface by chemical or physical treatment requires Supplementary engineering procedures. In the present study, regulation of a cell signal transduction pathway reversed the low Cellular activity on a hydrophobic surface without Surface modification. inhibition of Rho-associated kinase (ROCK) by Y-27632 markedly enhanced adhesion, Migration, and proliferation of osteoblastic cells cultured on a hydrophobic polystyrene Surface. ROCK inhibition regulated cell-cycle-related molecules on the hydrophobic Surface. This inhibition also decreased expression of the inhibitors of cyclin-dependent kinases such as p21(cip1) and p27(kip1) and increased expression of cyclin A and D. These results indicate that defective cellular activity on the hydrophobic Surface can be reversed by the control of a cell signal transduction pathway Without physicochemical surface modification. (C) 2009 Elsevier Inc. All rights reserved.
- ISSN
- 0006-291X
- Language
- English
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.