S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Effects of pilocarpine on the secretory acinar cells in human submandibular glands
- Li, Jinchao; Lee, Soojung; Choi, Se-Young; Lee, Jong-Ho; Chung, Sung Chang; Oh, Seog-Bae; Lee, Sung Joong; Kim, Joong-Soo; Park, Kyungpyo; Lee, Jong Heun
- Issue Date
- PERGAMON-ELSEVIER SCIENCE LTD
- LIFE SCIENCES, Vol.79, No.26, pp.2441-2447
- pilocarpine; ion channel; cytoplasmic free Ca2+ concentration ([Ca2+](i)); aquaporin-5; submandibular gland(SMG)
- Pilocarpine has been used as a choice of drugs for treatment of impaired salivary flow. Although considerable data are available as to the stimulatory effect of pilocarpine on the salivary secretion in human, its underlying mechanism, at the cellular level, has not been rigorously studied. In this experiment, we studied the effect of pilocarpine on the ion channel activity, cytoplasmic free Ca2+ concentration([Ca2+](i)) and aquaporin (AQP)-5 expression, which play key roles in the secretary process and determine the capacity of fluid secretion. In human submandibular gland (SMG) acinar cells, 10(-5) M pilocarpine activated the outward rectifying-current, which was predominantly K+ selective in the whole cell patch clamp study. The pilocarpine increased [Ca2+](i) in a concentration-dependent manner in the range of 10(-6) M to 10(-4) M. We found that both increases of [Ca2+](i) and outward rectifying-K+ current were inhibited by 10(-5) M U-73122, a specific phospholipase C inhibitor. The magnitudes of pilocarpine-induced [Ca2+](i) transients were approximately, 5 5 % lower than those with the same concentration of carbachol (CCh). Pilocarpine also increased the amount of AQP-5 protein in the apical membrane (APM) in human SMG acinar cells. Our results suggest that pilocarpine induce salivary secretions in human by activating K+ channels, increasing [Ca2+](i) via phospholipase C dependent pathway, and increasing AQP-5 protein expression in the APM, of SMG acinar cells. (c) 2006 Elsevier Inc. All rights reserved.
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