Browse

Odontogenic differentiation of human dental pulp stem cells induced by preameloblast-derived factors

DC Field Value Language
dc.contributor.authorLee, Ji-Hyun-
dc.contributor.authorLee, Dong-Seol-
dc.contributor.authorChoung, Han-Wool-
dc.contributor.authorShon, Won-Jun-
dc.contributor.authorLee, Eun-Hyang-
dc.contributor.authorPark, Joo-Cheol-
dc.contributor.authorCho, Je-Yoel-
dc.contributor.authorSeo, Byoung-Moo-
dc.date.accessioned2013-01-15T01:07:32Z-
dc.date.available2013-01-15T01:07:32Z-
dc.date.issued2011-12-
dc.identifier.citationBIOMATERIALS, Vol.32, No.36, pp.9696-9706ko_KR
dc.identifier.issn0142-9612-
dc.identifier.urihttps://hdl.handle.net/10371/80544-
dc.description.abstractThe differentiation of odontoblasts is initiated by the organization of differentiating ameloblasts during tooth formation. However, the exact roles of ameloblast-derived factors in odontoblast differentiation have not yet been characterized. We investigated the effects of preameloblast-conditioned medium (PACM) on the odontogenic differentiation of human dental pulp stem cells (hDPSCs) in vitro and in vivo. Furthermore, we analyzed the PA-CM by liquid chromatography-mass spectrometry to identify novel factors that facilitate odontoblast differentiation. In the co-culture of MDPC-23 cells or hDPSCs with mouse apical bud cells (ABCs), ABCs promoted differentiation of odontoblastic MDPC-23 cells and facilitated odontoblast differentiation of hDPSCs. PA-CM, CM from ABCs after 3 days culture, was most effective in increasing the dentin sialophosphoprotein promoter activity of odontoblastic MDPC-23 cells. When PA-CM-treated hDPSCs were transplanted into immunocompromised mice, they generated pulp-like structures lined with human odontoblast-like cells showing typical odontoblast processes. However, during recombinant human bone morphogenenetic protein 2-treated hDPSCs transplantation, some of the cells were entrapped in mineralized matrix possessing osteocyte characteristics. After proteomic analyses, we identified 113 types of proteins in PA-CM, of which we characterized 23. The results show that preameloblast-derived factors induce the odontogenic differentiation of hDPSCs and promote dentin formation. (C) 2011 Elsevier Ltd. All rights reserved.ko_KR
dc.description.sponsorshipThis study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry of Health & Welfare, Republic of
Korea (A101578).
ko_KR
dc.language.isoenko_KR
dc.publisherELSEVIERko_KR
dc.subjectEpithelial-mesenchymal interactionko_KR
dc.subjectPreameloblast-CMko_KR
dc.subjectHuman dental pulp stem cellko_KR
dc.subjectDentin regenerationko_KR
dc.subjectOdontoblast differentiationko_KR
dc.titleOdontogenic differentiation of human dental pulp stem cells induced by preameloblast-derived factorsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이지현-
dc.contributor.AlternativeAuthor이동설-
dc.contributor.AlternativeAuthor정한울-
dc.contributor.AlternativeAuthor손원준-
dc.contributor.AlternativeAuthor이은향-
dc.contributor.AlternativeAuthor박주철-
dc.contributor.AlternativeAuthor조제열-
dc.contributor.AlternativeAuthor서병무-
dc.identifier.doi10.1016/j.biomaterials.2011.09.007-
dc.citation.journaltitleBIOMATERIALS-
dc.description.tc1-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse