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Odontogenic differentiation of human dental pulp stem cells induced by preameloblast-derived factors
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Ji-Hyun | - |
dc.contributor.author | Lee, Dong-Seol | - |
dc.contributor.author | Choung, Han-Wool | - |
dc.contributor.author | Shon, Won-Jun | - |
dc.contributor.author | Lee, Eun-Hyang | - |
dc.contributor.author | Park, Joo-Cheol | - |
dc.contributor.author | Cho, Je-Yoel | - |
dc.contributor.author | Seo, Byoung-Moo | - |
dc.date.accessioned | 2013-01-15T01:07:32Z | - |
dc.date.available | 2013-01-15T01:07:32Z | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | BIOMATERIALS, Vol.32, No.36, pp.9696-9706 | ko_KR |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://hdl.handle.net/10371/80544 | - |
dc.description.abstract | The differentiation of odontoblasts is initiated by the organization of differentiating ameloblasts during tooth formation. However, the exact roles of ameloblast-derived factors in odontoblast differentiation have not yet been characterized. We investigated the effects of preameloblast-conditioned medium (PACM) on the odontogenic differentiation of human dental pulp stem cells (hDPSCs) in vitro and in vivo. Furthermore, we analyzed the PA-CM by liquid chromatography-mass spectrometry to identify novel factors that facilitate odontoblast differentiation. In the co-culture of MDPC-23 cells or hDPSCs with mouse apical bud cells (ABCs), ABCs promoted differentiation of odontoblastic MDPC-23 cells and facilitated odontoblast differentiation of hDPSCs. PA-CM, CM from ABCs after 3 days culture, was most effective in increasing the dentin sialophosphoprotein promoter activity of odontoblastic MDPC-23 cells. When PA-CM-treated hDPSCs were transplanted into immunocompromised mice, they generated pulp-like structures lined with human odontoblast-like cells showing typical odontoblast processes. However, during recombinant human bone morphogenenetic protein 2-treated hDPSCs transplantation, some of the cells were entrapped in mineralized matrix possessing osteocyte characteristics. After proteomic analyses, we identified 113 types of proteins in PA-CM, of which we characterized 23. The results show that preameloblast-derived factors induce the odontogenic differentiation of hDPSCs and promote dentin formation. (C) 2011 Elsevier Ltd. All rights reserved. | ko_KR |
dc.description.sponsorship | This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry of Health & Welfare, Republic of
Korea (A101578). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | ELSEVIER | ko_KR |
dc.subject | Epithelial-mesenchymal interaction | ko_KR |
dc.subject | Preameloblast-CM | ko_KR |
dc.subject | Human dental pulp stem cell | ko_KR |
dc.subject | Dentin regeneration | ko_KR |
dc.subject | Odontoblast differentiation | ko_KR |
dc.title | Odontogenic differentiation of human dental pulp stem cells induced by preameloblast-derived factors | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이지현 | - |
dc.contributor.AlternativeAuthor | 이동설 | - |
dc.contributor.AlternativeAuthor | 정한울 | - |
dc.contributor.AlternativeAuthor | 손원준 | - |
dc.contributor.AlternativeAuthor | 이은향 | - |
dc.contributor.AlternativeAuthor | 박주철 | - |
dc.contributor.AlternativeAuthor | 조제열 | - |
dc.contributor.AlternativeAuthor | 서병무 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2011.09.007 | - |
dc.citation.journaltitle | BIOMATERIALS | - |
dc.description.tc | 1 | - |
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