Publications
Detailed Information
Resveratrol suppresses growth of human ovarian cancer cells in culture and in a murine xenograft model: Eukaryotic elongation factor 1A2 as a potential target
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Mee-Hyun | - |
dc.contributor.author | Choi, Bu Young | - |
dc.contributor.author | Kundu, Joydeb Kumar | - |
dc.contributor.author | Shin, Young Kee | - |
dc.contributor.author | Na, Hye-Kyung | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2013-01-22T02:27:11Z | - |
dc.date.available | 2013-01-22T02:27:11Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2009-10 | - |
dc.identifier.citation | Cancer Research, Vol.69 No.18, pp.7449-7458 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.other | 2805 | - |
dc.identifier.uri | https://hdl.handle.net/10371/80932 | - |
dc.description.abstract | The eukaryotic elongation factor 1A2 (eEF1A2) is known to retain oncogenic potential and is recognized as a novel target for cancer prevention and therapy. Resveratrol (trans-3,4',5-trihydroxystilbene), a phytoalexin present in grapes, has been reported to possess chemopreventive and chemotherapeutic activities. In the present study, we examined the growth-inhibitory effects of resveratrol in human ovarian cancer PA-1 cells, considering eEF1A2 as a potential molecular target. Pretreatment with resveratrol attenuated proliferation of serum-starved PA-1. cells stimulated with insulin or serum. Resveratrol also activated caspase-9, -7, and -3 and induced apoptosis in PA-1 cells in the presence of insulin or serum. Insulin or serum stimulation of PA-1 cells resulted in the marked induction of eEF1A2, which was suppressed by pretreatment with resveratrol. Moreover, resveratrol inhibited insulin- or serum-induced soft-agar colony formation in eEF1A2-transfected NIH3T3 cells. An antibody array directed to assess the phosphorylation of protein kinases revealed that treatment with insulin or serum induced the phosphorylation of Akt in PA-1 cells. Pharmacologic inhibition of Akt with LY294002 abrogated insulin- or serum-induced eEF1A2 expression and increased the caspase-3 activity. In another experiment, i.p. administration of resveratrol retarded the growth of PA-1 cell xenograft and the expression of eEF1A2 in athymic nude mice in association with decreased bromodeoxyuridine positivity, reduced expression of proliferating cell nuclear antigen, increased the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and caspase-3 staining, and diminished CD31 positivity. Taken together, eEF1A2 may he considered as a potential molecular target for the antiproliferative effects of resveratrol in PA-1 ovarian cancer cells. [Cancer Res 2009;69(18):7449-58] | - |
dc.language | 영어 | - |
dc.language.iso | en | ko_KR |
dc.publisher | American Association for Cancer Research | - |
dc.title | Resveratrol suppresses growth of human ovarian cancer cells in culture and in a murine xenograft model: Eukaryotic elongation factor 1A2 as a potential target | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 서영준 | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-09-1266 | - |
dc.citation.journaltitle | Cancer Research | - |
dc.description.tc | 21 | - |
dc.identifier.wosid | 000269954000040 | - |
dc.identifier.scopusid | 2-s2.0-70349736107 | - |
dc.citation.endpage | 7458 | - |
dc.citation.number | 18 | - |
dc.citation.startpage | 7449 | - |
dc.citation.volume | 69 | - |
dc.identifier.sci | 000269954000040 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Shin, Young Kee | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | FACTOR EEF1A2 | - |
dc.subject.keywordPlus | COLON-CANCER | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | INSULIN | - |
dc.subject.keywordPlus | LINES | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | CHEMOPREVENTION | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.