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Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2

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dc.contributor.authorHan, Seung-Hee-
dc.contributor.authorKim, Kyoung-Hwa-
dc.contributor.authorHan, Jung-Seok-
dc.contributor.authorKoo, Ki-Tae-
dc.contributor.authorSeol, Yang-Jo-
dc.contributor.authorKu, Young-
dc.contributor.authorRhyu, In-Chul-
dc.contributor.authorLee, Yong-Moo-
dc.contributor.authorKim, Tae-Il-
dc.date.accessioned2013-01-23T01:26:02Z-
dc.date.available2013-01-23T01:26:02Z-
dc.date.issued2011-
dc.identifier.citationJournal of Periodontal and Implant Science; Vol.41, No.5, pp.227-233ko_KR
dc.identifier.issn2093-2278-
dc.identifier.urihttps://hdl.handle.net/10371/81011-
dc.description.abstractPurpose: The aim of this study was to compare osteoblast behavior on zirconia and titanium under conditions cultured with bone morphogenetic protein-2. Methods: MC 3T 3-E1 cells were cultured on sandblasted zirconia and sandblasted/etched titanium discs. At 24 hours after seeding MC 3T 3-E1, the demineralized bone matrix (DBM) gel alone and the DBM gel with bone morphogenetic protein-2 (BMP-2) were added to the culture medium. The surface topography was examined by confocal laser scanning microscopy. Cellular proliferation was measured at 1, 4, and 7 days after gel loading. Alkaline phosphatase activity was measured at 7 days after gel loading. The mRNA expression of ALPase, bone sialoprotein, type I collagen, runt-related transcription factor 2 (Runx-2), osteocalcin, and osterix were evaluated by real-time polymerase chain reaction at 4 days and 7 days. Results: At 1, 4, and 7 days after loading the DBM gel alone and the DBM gel with BMP-2, cellular proliferation on the zirconia and titanium discs was similar and that of the groups cultured with the DBM gel alone and the DBM gel with BMP-2 was not significantly different, except for titanium with BMP-2 gel. ALPase activity was higher in the cells cultured with BMP-2 than in the other groups, but there was no difference between the zirconia and titanium. In ALPase, bone sialoprotein, osteocalcin, Runx-2 and osterix gene expression, that of cells on zirconia or titanium with BMP-2 gel was much more highly increased than titanium without gel at day 7. The gene expression level of cells cultured on zirconia with BMP-2 was higher than that on titanium with BMP-2 at day 7. Conclusions: The data in this study demonstrate that the osteoblastic cell attachment and proliferation of zirconia were comparable to those of titanium. With the stimulation of BMP-2, zirconia has a more pronounced effect on the proliferation and differentiation of the osteoblastic cells compared with titanium. ⓒ 2011 Korean Academy of Periodontology.ko_KR
dc.language.isoenko_KR
dc.publisherKorean Academy of Periodontologyko_KR
dc.subjectBone morphogenetic protein-2ko_KR
dc.subjectCell differentiationko_KR
dc.subjectZirconium oxideko_KR
dc.subjectCell proliferationko_KR
dc.titleResponse of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2ko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor한승희-
dc.contributor.AlternativeAuthor김경화-
dc.contributor.AlternativeAuthor한중석-
dc.contributor.AlternativeAuthor구기태-
dc.contributor.AlternativeAuthor설양조-
dc.contributor.AlternativeAuthor구영-
dc.contributor.AlternativeAuthor류인철-
dc.contributor.AlternativeAuthor이용무-
dc.contributor.AlternativeAuthor김태일-
dc.identifier.doi10.5051/jpis.2011.41.5.227-
dc.citation.journaltitleJournal of Periodontal and Implant Science-
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