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Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Seung-Hee | - |
dc.contributor.author | Kim, Kyoung-Hwa | - |
dc.contributor.author | Han, Jung-Seok | - |
dc.contributor.author | Koo, Ki-Tae | - |
dc.contributor.author | Seol, Yang-Jo | - |
dc.contributor.author | Ku, Young | - |
dc.contributor.author | Rhyu, In-Chul | - |
dc.contributor.author | Lee, Yong-Moo | - |
dc.contributor.author | Kim, Tae-Il | - |
dc.date.accessioned | 2013-01-23T01:26:02Z | - |
dc.date.available | 2013-01-23T01:26:02Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Journal of Periodontal and Implant Science; Vol.41, No.5, pp.227-233 | ko_KR |
dc.identifier.issn | 2093-2278 | - |
dc.identifier.uri | https://hdl.handle.net/10371/81011 | - |
dc.description.abstract | Purpose: The aim of this study was to compare osteoblast behavior on zirconia and titanium under conditions cultured with bone morphogenetic protein-2. Methods: MC 3T 3-E1 cells were cultured on sandblasted zirconia and sandblasted/etched titanium discs. At 24 hours after seeding MC 3T 3-E1, the demineralized bone matrix (DBM) gel alone and the DBM gel with bone morphogenetic protein-2 (BMP-2) were added to the culture medium. The surface topography was examined by confocal laser scanning microscopy. Cellular proliferation was measured at 1, 4, and 7 days after gel loading. Alkaline phosphatase activity was measured at 7 days after gel loading. The mRNA expression of ALPase, bone sialoprotein, type I collagen, runt-related transcription factor 2 (Runx-2), osteocalcin, and osterix were evaluated by real-time polymerase chain reaction at 4 days and 7 days. Results: At 1, 4, and 7 days after loading the DBM gel alone and the DBM gel with BMP-2, cellular proliferation on the zirconia and titanium discs was similar and that of the groups cultured with the DBM gel alone and the DBM gel with BMP-2 was not significantly different, except for titanium with BMP-2 gel. ALPase activity was higher in the cells cultured with BMP-2 than in the other groups, but there was no difference between the zirconia and titanium. In ALPase, bone sialoprotein, osteocalcin, Runx-2 and osterix gene expression, that of cells on zirconia or titanium with BMP-2 gel was much more highly increased than titanium without gel at day 7. The gene expression level of cells cultured on zirconia with BMP-2 was higher than that on titanium with BMP-2 at day 7. Conclusions: The data in this study demonstrate that the osteoblastic cell attachment and proliferation of zirconia were comparable to those of titanium. With the stimulation of BMP-2, zirconia has a more pronounced effect on the proliferation and differentiation of the osteoblastic cells compared with titanium. ⓒ 2011 Korean Academy of Periodontology. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Korean Academy of Periodontology | ko_KR |
dc.subject | Bone morphogenetic protein-2 | ko_KR |
dc.subject | Cell differentiation | ko_KR |
dc.subject | Zirconium oxide | ko_KR |
dc.subject | Cell proliferation | ko_KR |
dc.title | Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2 | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 한승희 | - |
dc.contributor.AlternativeAuthor | 김경화 | - |
dc.contributor.AlternativeAuthor | 한중석 | - |
dc.contributor.AlternativeAuthor | 구기태 | - |
dc.contributor.AlternativeAuthor | 설양조 | - |
dc.contributor.AlternativeAuthor | 구영 | - |
dc.contributor.AlternativeAuthor | 류인철 | - |
dc.contributor.AlternativeAuthor | 이용무 | - |
dc.contributor.AlternativeAuthor | 김태일 | - |
dc.identifier.doi | 10.5051/jpis.2011.41.5.227 | - |
dc.citation.journaltitle | Journal of Periodontal and Implant Science | - |
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