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Tissue Engineered Bone Formation Using Chitosan/Tricalcium Phosphate Sponges

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dc.contributor.authorLee, Yong-Moo-
dc.contributor.authorPark, Yoon-Jeong-
dc.contributor.authorLee, Seung-Jin-
dc.contributor.authorKu, Young-
dc.contributor.authorChoi, Sang-Mook-
dc.contributor.authorChung, Chong-Pyoung-
dc.contributor.authorKlokkevold, Perry R.-
dc.contributor.authorHan, Soo-Boo-
dc.date.accessioned2013-01-23T06:46:44Z-
dc.date.available2013-01-23T06:46:44Z-
dc.date.issued2000-
dc.identifier.citationJournal of Periodontology, Vol.71, No.3, pp.410-417ko_KR
dc.identifier.issn0022-3492-
dc.identifier.urihttps://hdl.handle.net/10371/81045-
dc.description.abstractBackground: Chitosan is a biodegradable natural polymer that has been shown to improve wound healing. This study aimed to develop chitosan/tricalcium phosphate (TCP) sponges as tissue engineering scaffolds for bone formation by three-dimensional osteoblast culture. Methods: The sponges were prepared by freeze-drying and cross-linking a mixture of chitosan solution with TCP. Fetal rat calvarial osteoblastic cells were isolated, cultured, and seeded into the sponges. The cell-sponge constructs were cultured for 56 days. Cell proliferation, alkaline phosphatase (ALPase) activity, and calcium deposition in the cell-sponge constructs were measured at 1, 7, 14, 28, and 56 days. Histologic examination was performed with light microscopy and scanning electron microscopy. Results: Chitosan/TCP sponges supported the proliferation of osteoblastic cells as well as their differentiation as indicated by high ALPase activities and deposition of mineralized matrices by the cells. Light and scanning electron microscopic examination indicated that seeded osteoblastic cells were well attached to sponge matrices and proliferated in a multi-layer fashion. Small bone-like spicules were observed on the sponge matrix at 14 days. Seeded cells appeared to be embedded in the newly formed tissue matrix, which is characteristic of the osteoblast differentiation and their progression into osteocytic cells. The amount of mineralized tissue formed in the sponge at 56 days was significant. Conclusions: These results suggest that the chitosan/TCP sponge is a feasible tool as a scaffolding material to grow osteoblast in a three-dimensional structure for transplantation into a site for bone regeneration.ko_KR
dc.language.isoenko_KR
dc.publisherAmerican Academy of Periodontologyko_KR
dc.subjectAnimal studiesko_KR
dc.subjectArtificialko_KR
dc.subjectBone developmentko_KR
dc.subjectSpongesko_KR
dc.subjectTricalcium phosphateko_KR
dc.subjectWound healingko_KR
dc.subjectTissue engineeringko_KR
dc.subjectOsteoblastsko_KR
dc.subjectChitosanko_KR
dc.subjectBone regenerationko_KR
dc.titleTissue Engineered Bone Formation Using Chitosan/Tricalcium Phosphate Spongesko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이용무-
dc.contributor.AlternativeAuthor박윤정-
dc.contributor.AlternativeAuthor이승진-
dc.contributor.AlternativeAuthor구영-
dc.contributor.AlternativeAuthor최상묵-
dc.contributor.AlternativeAuthor정종평-
dc.contributor.AlternativeAuthor한수부-
dc.identifier.doi10.1902/jop.2000.71.3.410-
dc.citation.journaltitleJournal of Periodontology-
dc.description.tc72-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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