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Suberoylanilide Hydroxamic Acid Enhances Odontoblast Differentiation

Cited 16 time in Web of Science Cited 16 time in Scopus
Authors
Kwon, A.; Park, H.-J.; Baek, K.; Lee, H.-L.; Park, J.-C.; Woo, K.M.; Ryoo, H.-M.; Baek, J.-H.
Issue Date
2012-03
Publisher
SAGE Publications
Citation
JOURNAL OF DENTAL RESEARCH Vol.91 No.5, pp. 506-512
Keywords
복합학SAHANficMDPC23 cellstranscriptionpromoter
Abstract
Previous studies have shown that histone deacetylase
(HDAC) inhibitors stimulate osteoblast differentiation
in vitro and bone formation in vivo.
However, the effects of HDAC inhibitors on odontoblasts
have not been elucidated. Therefore, in
this study, we examined the effect of suberoylanilide
hydroxamic acid (SAHA), an HDAC inhibitor,
on odontoblast differentiation using an
MDPC23 odontoblast-like cell line. SAHA significantly
enhanced matrix mineralization and the
expression levels of odontoblast marker genes.
SAHA increased the expression levels of nuclear
factor I/C (Nfic) and dentin sialophosphoprotein
(Dspp). Nfic bound directly to the Dspp promoter
and stimulated Dspp transcription. SAHA
increased both basal and Nfic-induced Dspp promoter
activity. SAHA-induced Dspp promoter
activity disappeared when mutations were introduced
within the Nfic binding element of the Dspp
promoter. Nfic knockdown by siRNA blocked
SAHA stimulation of Dspp expression. These
results indicate that SAHA enhances odontoblast
differentiation and that SAHA increases Dspp
expression, at least in part, by increasing the
expression level of Nfic.
Previous studies have shown that histone deacetylase
(HDAC) inhibitors stimulate osteoblast differentiation
in vitro and bone formation in vivo.
However, the effects of HDAC inhibitors on odontoblasts
have not been elucidated. Therefore, in
this study, we examined the effect of suberoylanilide
hydroxamic acid (SAHA), an HDAC inhibitor,
on odontoblast differentiation using an
MDPC23 odontoblast-like cell line. SAHA significantly
enhanced matrix mineralization and the
expression levels of odontoblast marker genes.
SAHA increased the expression levels of nuclear
factor I/C (Nfic) and dentin sialophosphoprotein
(Dspp). Nfic bound directly to the Dspp promoter
and stimulated Dspp transcription. SAHA
increased both basal and Nfic-induced Dspp promoter
activity. SAHA-induced Dspp promoter
activity disappeared when mutations were introduced
within the Nfic binding element of the Dspp
promoter. Nfic knockdown by siRNA blocked
SAHA stimulation of Dspp expression. These
results indicate that SAHA enhances odontoblast
differentiation and that SAHA increases Dspp
expression, at least in part, by increasing the
expression level of Nfic.
ISSN
0022-0345
Language
English
URI
https://hdl.handle.net/10371/81395
DOI
https://doi.org/10.1177/0022034512443367
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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