흰 쥐의 생체밖 소장의 수분 및 전해질 흡수

Abstract

Isolated intestinal loops of 54 rats were perfused with Krebs·Ringer·bicarbonate-glucose solution under pressure of 10 or 25 cmH20 at a constant temperature of 40°C. Among them some loops were everted and the serosal to mucosal transport was also studied. The perfusing solution was saturated with 95%02 + 5%C20. Samples collected in the bottom of the perfusing apparatus were taken with one hour interval. Following conclusions were made. 1. The amount of fluid transferred related to the pressure applied and regional differences were noticed, e.g.. more fluid passed through the jejunal wall than through the ileum in unit time. 2. In the everted loops less amount of fluid was transferred than in the uneverted preparations. 3. The osmolality of the transferred fluid was slightly higher than that of the perfusing fluid, suggesting some addition of solutes in the course of the passage. 4. Comparingthe constituents of the perfusing and the transferred fluids, following results were revealed. a. Transferred fluid had lower concentrations of sodium, potassium and urea in comparison with those of the perfusing fluid. b. Unique characteristics of SCN- was observed in the transport through the wall of ileum, having a higher concentration in the transferred fluid than in the perfusing fluid. Combining this fact with the lower concentration of SCNin the ileal wall the presence of organic anions in abundance in the tissue was suggested.