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Effects of GM-CSF gene transfer using silica-nanoparticles as a vehicle on white blood cell production in dogs

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dc.contributor.authorChoi, Eun Wha-
dc.contributor.authorShin, Il Seob-
dc.contributor.authorChae, Young Jin-
dc.contributor.authorKoo, Hye Cheong-
dc.contributor.authorLee, Jong Hwa-
dc.contributor.authorChung, Tae Ho-
dc.contributor.authorPark, Yong Ho-
dc.contributor.authorKim, Dae-Yong-
dc.contributor.authorHwang, Cheol Yong-
dc.contributor.authorLee, Chang Woo-
dc.contributor.authorYoun, Hwa Young-
dc.date.accessioned2009-08-31T23:26:34Z-
dc.date.available2009-08-31T23:26:34Z-
dc.date.issued2008-04-02-
dc.identifier.citationExp Hematol. 2008;36:807-815en
dc.identifier.issn0301-472X-
dc.identifier.urihttps://hdl.handle.net/10371/8280-
dc.description.abstractObjective. We sought to test two concepts: that nanoparticles can be used for in vivo gene
delivery and that canine granulocyte-macrophage colony-stimulating factor (GM-CSF)/nanoparticles
can have possibility to be used to treat transient (acute) canine leukopenia.
Materials and Methods. We have generated a novel fluorescent-silica nanoparticle binding of
canine GM-CSF gene; canine GM-CSF gene was inserted between the cytomegalovirus promoter
and poly-adenylation sequences of simian virus 40, and the gene construct was ligated
to fluorescent silica nanoparticles functionalized with tertiary amine.
Results. When the GM-CSF/nanoparticles were injected into normal dogs, the GM-CSF was
expressed in peripheral blood mononuclear cells for at least 9 days and there were significant
increases in white blood cell counts, as confirmed by complete blood count, differential count,
and flow cytometry. Significant increases in expression of major histocompatibility complex
class II on granulocytes and in serum GM-CSF were also observed. Readministration of
the nanoparticles was also effective and expression in various tissues was confirmed by reverse
transcriptase polymerase chain reaction.
Conclusions. These GM-CSF/nanoparticles may be useful for correction of acute leukopenia,
such as chemotherapy-induced myelosuppression without developing neutralizing antibodies.
en
dc.description.sponsorshipWe would like to thank Sook Shin (Department of Veterinary Microbiology)
for her outstanding technical assistance. This work
was supported by the Korea Research Foundation Grant funded
by the Korean Government (MOEHRD) (KRF-2006-311-
E00526). Further support was also provided by other Korea
Research Foundation Grants (KRF-2006-005-J02902 and KRF-
2006-005-J02903) and the Research Institute of Veterinary Science,
College of Veterinary Medicine, Seoul National University
and the Brain Korea 21 Program for Veterinary Science.
en
dc.language.isoen-
dc.publisherElsevieren
dc.titleEffects of GM-CSF gene transfer using silica-nanoparticles as a vehicle on white blood cell production in dogsen
dc.typeArticleen
dc.contributor.AlternativeAuthor최은화-
dc.contributor.AlternativeAuthor신일섭-
dc.contributor.AlternativeAuthor채영진-
dc.contributor.AlternativeAuthor구혜정-
dc.contributor.AlternativeAuthor이종화-
dc.contributor.AlternativeAuthor정태호-
dc.contributor.AlternativeAuthor박용호-
dc.contributor.AlternativeAuthor김대용-
dc.contributor.AlternativeAuthor황철용-
dc.contributor.AlternativeAuthor이창우-
dc.contributor.AlternativeAuthor윤화영-
dc.identifier.doi10.1016/j.exphem.2008.01.007-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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