Publications
Detailed Information
Effects of GM-CSF gene transfer using silica-nanoparticles as a vehicle on white blood cell production in dogs
Cited 12 time in
Web of Science
Cited 14 time in Scopus
- Authors
- Issue Date
- 2008-04-02
- Publisher
- Elsevier
- Citation
- Exp Hematol. 2008;36:807-815
- Abstract
- Objective. We sought to test two concepts: that nanoparticles can be used for in vivo gene
delivery and that canine granulocyte-macrophage colony-stimulating factor (GM-CSF)/nanoparticles
can have possibility to be used to treat transient (acute) canine leukopenia.
Materials and Methods. We have generated a novel fluorescent-silica nanoparticle binding of
canine GM-CSF gene; canine GM-CSF gene was inserted between the cytomegalovirus promoter
and poly-adenylation sequences of simian virus 40, and the gene construct was ligated
to fluorescent silica nanoparticles functionalized with tertiary amine.
Results. When the GM-CSF/nanoparticles were injected into normal dogs, the GM-CSF was
expressed in peripheral blood mononuclear cells for at least 9 days and there were significant
increases in white blood cell counts, as confirmed by complete blood count, differential count,
and flow cytometry. Significant increases in expression of major histocompatibility complex
class II on granulocytes and in serum GM-CSF were also observed. Readministration of
the nanoparticles was also effective and expression in various tissues was confirmed by reverse
transcriptase polymerase chain reaction.
Conclusions. These GM-CSF/nanoparticles may be useful for correction of acute leukopenia,
such as chemotherapy-induced myelosuppression without developing neutralizing antibodies.
- ISSN
- 0301-472X
- Language
- English
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.