S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
Poly(ester amine)-mediated, aerosol-delivered Akt1 small interfering RNA suppresses lung tumorigenesis
- Xu, Cheng-Xiong; Jere, Dhananjay; Jin, Hua; Chang, Seung-Hee; Chung, Youn-Sun; Shin, Ji-Young; Kim, Ji-Eun; Park, Sung-Jin; Lee, Yong-Hoon; Chae, Chan-Hee; Lee, Kee Ho; Beck, George R., Jr.; Cho, Chong-Su; Cho, Myung-Haing
- Issue Date
- American Thoracic Society
- American Journal of Respiratory and Critical Care Medicine, Vol.178 No.1, pp.60-73
- poly(ester amine); Akt1 siRNA; lung cancer; K-ras(LA1) mice; urethane; aerosol gene delivery
- Rationale: The low efficiency of conventional therapies in achieving long-term survival of patients with lung cancer calls for the development of novel therapeutic options. Recent advances in aerosol-mediated gene delivery have provided the possibility of an alternative for the safe and effective treatment of lung cancer. Objectives: To demonstrate the feasibility and emphasize the importance of noninvasive aerosol delivery of Akt1 small interfering RNA (siRNA) as an effective and selective option for lung cancer treatment. Methods: Nanosized poly(ester amine) polymer was synthesized and used as a gene carrier. An aerosol of poly(ester amine)/Akt1 siRNA complex was delivered into K-ras(LA1) and urethane-induced lung cancer models through a nose-only inhalation system. The effects of Akt1 siRNA on lung cancer progression and Akt-related signals were evaluated. Measurements and Main Results: The aerosol-delivered Akt1 siRNA suppressed lung tumor progression significantly through inhibiting Akt-related signals and cell cycle, Conclusions: The use of poly(ester amine) serves as an effective carrier, and aerosol delivery of Akt1 siRNA may be a promising approach for lung cancer treatment and prevention.
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