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The effect of gene therapy using CTLA4Ig/silica-nanoparticles on canine experimental autoimmune thyroiditis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Eun Wha | - |
dc.contributor.author | Shin, Il Seob | - |
dc.contributor.author | Lee, Chang Woo | - |
dc.contributor.author | Youn, Hwa Young | - |
dc.date.accessioned | 2009-08-31T23:29:57Z | - |
dc.date.available | 2009-08-31T23:29:57Z | - |
dc.date.issued | 2008-05-02 | - |
dc.identifier.citation | J Gene Med 2008; 10: 795-804 | en |
dc.identifier.issn | 1099-498X | - |
dc.identifier.uri | https://hdl.handle.net/10371/8284 | - |
dc.description.abstract | Background The present study aimed to determine the effect of canine
CTLA4Ig on canine autoimmune thyroiditis. In a previous study, we established a canine model of autoimmune thyroiditis by immunizing normal dogs with bovine thyroglobulin. An in vitro study using recombinant CTLA4Ig revealed that this protein can inhibit the expression of Th1-type cytokines and the pro-inflammatory cytokines tested. Methods As a result of the in vitro study, we constructed therapeutic CTLA4Ig/silica-nanoparticles and applied them to the treatment of experimentally induced canine autoimmune thyroiditis. Results Gene therapy resulted in significant reductions in anti-caninethyroglobulin autoantibody titer, anti-T4 antibody titer and T-cell proliferation against thyroglobulin and in the mRNA expressions of interleukin-18 in fresh peripheral blood mononuclear cells (PBMC) from all dogs. There was also a significant reduction compared to day 0 in tumor necrosis factor-α and interferon-γ levels in the supernatant from cultured PBMC. Conclusions The CTLA4Ig-induced suppression of Th1 cytokines is relatively more significant than it appears because autoimmune thyroiditis is a Th1-polarized disease. Thus, CTLA4Ig can improve Th1/Th2 cytokine balance in autoimmune thyroiditis by downregulating Th1 cytokines. | en |
dc.description.sponsorship | The authors thank Young Jin Chae (Biterials Co.) for freely
providing nanoparticles, and the authors thank the staff of the Department of Veterinary Pathology. This work was supported by Korean Research Foundation Grant (KRF-2006- 005-J02902). Further support was provided by the Research Institute of Veterinary Science, College of Veterinary Medicine, Seoul National University and the Brain Korea 21 Program for Veterinary Science. | en |
dc.language.iso | en | en |
dc.publisher | Wiley-Blackwell | en |
dc.subject | autoimmune thyroiditis | en |
dc.subject | CTLA4Ig | en |
dc.subject | cytokine | en |
dc.subject | dog | en |
dc.subject | semi-quantitative | en |
dc.subject | RT-PCR | en |
dc.subject | T cell proliferation | en |
dc.title | The effect of gene therapy using CTLA4Ig/silica-nanoparticles on canine experimental autoimmune thyroiditis | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 최은화 | - |
dc.contributor.AlternativeAuthor | 신일섭 | - |
dc.contributor.AlternativeAuthor | 이창우 | - |
dc.contributor.AlternativeAuthor | 윤화영 | - |
dc.identifier.doi | 10.1002/jgm.1203 | - |
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