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Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Eun-Jung | - |
dc.contributor.author | Yi, Jongheop | - |
dc.contributor.author | Chung, Kyu-Hyuck | - |
dc.contributor.author | Ryu, Doug Young | - |
dc.contributor.author | Choi, Jinhee | - |
dc.contributor.author | Park, Kwangsik | - |
dc.date.accessioned | 2009-08-31T23:40:07Z | - |
dc.date.available | 2009-08-31T23:40:07Z | - |
dc.date.issued | 2008-07-10 | - |
dc.identifier.citation | Toxicol. Lett. 180, 222-229 | en |
dc.identifier.issn | 0378-4274 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8291 | - |
dc.description.abstract | As the applications of industrial nanoparticles are being developed, the concerns on the environmental
health are increasing. Cytotoxicities of titanium dioxide nanoparticles of different concentrations (5, 10, 20 and 40 g/ml) were evaluated in this study using a cultured human bronchial epithelial cell line, BEAS-2B. Exposure of the cultured cells to nanoparticles led to cell death, reactive oxygen species (ROS) increase, reduced glutathione (GSH) decrease, and the induction of oxidative stress-related genes such as heme oxygenase-1, thioredoxin reductase, glutathione-S-transferase, catalase, and a hypoxia inducible gene. The ROS increase by titanium dioxide nanoparticles triggered the activation of cytosolic caspase- 3 and chromatin condensation, which means that titanium dioxide nanoparticles exert cytotoxicity by an apoptotic process. Furthermore, the expressions of inflammation-related genes such as interleukin- 1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), TNF-a, and C-X-C motif ligand 2 (CXCL2) were also elevated. The induction of IL-8 by titanium dioxide nanoparticles was inhibited by the pre-treatment with SB203580 and PD98059, which means that the IL-8 was induced through p38 mitogen-acitvated protein kinase (MAPK) pathway and/or extracellular signal (ERK) pathway. Uptake of the nanoparticles into the cultured cells was observed and titanium dioxide nanoparticles seemed to penetrate into the cytoplasm and locate in the peri-region of the nucleus as aggregated particles, which may induce direct interactions between the particles and cellular molecules, to cause adverse biological responses. | en |
dc.description.sponsorship | Thisworkwas supported by the Eco-technopia 21 project of the
Korea Ministry of the Environment. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Titanium dioxide nanoparticles | en |
dc.subject | Apoptosis | en |
dc.subject | Oxidative stress | en |
dc.subject | BEAS-2B cells | en |
dc.title | Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박은정 | - |
dc.contributor.AlternativeAuthor | 이종협 | - |
dc.contributor.AlternativeAuthor | 정규혁 | - |
dc.contributor.AlternativeAuthor | 류덕영 | - |
dc.contributor.AlternativeAuthor | 최진희 | - |
dc.contributor.AlternativeAuthor | 박광식 | - |
dc.identifier.doi | 10.1016/j.toxlet.2008.06.869 | - |
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