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Electrical activation induces reactive oxygen species in porcine embryos

Cited 24 time in Web of Science Cited 25 time in Scopus
Authors
Koo, O.J.; Jang, Goo; Kwon, D.K.; Kang, J.T.; Kwon, O.S.; Park, H.J.; Kang, S.K.; Lee, Byeong Chun
Issue Date
2008-08-01
Publisher
Elsevier
Citation
Theriogenology 2008;70:1111-8
Keywords
Electrical activationROSEmbryoGSH6-DMAP
Abstract
The objectives were to determine factors affecting generation of reactive oxygen species (ROS) in porcine embryos after electrical activation of oocytes, and the effects of an antioxidant and chemical agent on ROS generation. Greater ROS were induced by electrical activation compared to IVF (mean ± S.E.M., 14.6 ± 0.8 vs. 9.2 ± 0.4, P < 0.05). Furthermore, ROS generation in embryos after electrical activation was significantly increased by higher intensity and longer duration electrical pulses and by higher exogenous Ca2+ concentrations. Cleavage rate and blastocyst formation rate were not directly related to the level of ROS. Supplementation of the IVC medium with 0.5 mM glutathione (GSH) reduced ROS (9.2 ± 0.4 vs. 14.7 ± 0.9, P < 0.05). Treatment with the chemical activation agent, 6-dimethylaminopurine (6-DMAP) for 3 h did not induce further ROS generation in combination with electrical activation, but it improved blastocyst formation rate (53.8 ± 1.1 vs. 23.7 ± 3.5, P < 0.05). We concluded that generation of ROS should be considered for optimizing electrical activation and that supplementing an antioxidant or combining electrical and chemical activation induced lower ROS generation in electrically activated porcine embryos.
ISSN
0093-691X
Language
English
URI
https://hdl.handle.net/10371/8316
DOI
https://doi.org/10.1016/j.theriogenology.2008.06.031
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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