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A protein delivery system using 30Kc19 cell-penetrating protein originating from silkworm

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Authors
Park, Ju Hyun; Lee, Ju Hyun; Park, Hee Ho; Rhee, Won Jong; Choi, Shin Sik; Park, Tai Hyun
Issue Date
2012-12
Publisher
Elsevier Ltd
Citation
Biomaterials Vol.33 No.35, pp. 9127-9134
Keywords
복합학30Kc19 proteinCell-penetrating proteinProtein transduction domainProtein deliveryIn vivo transduction
Abstract
Cell-penetrating protein and its protein transduction domain have been used to deliver drugs and proteins into the cells via receptor-independent endocytosis. A number of cell-penetrating proteins including TAT derived from HIV-1 virus, VP22 from herpes simplex virus and Antennapedia from drosophila have been discovered. Here, we report a cell-penetrating protein, 30Kc19, originating from the hemolymph of silkworm, Bombyx mori. The 30Kc19 is the first cell-penetrating protein that has been found in insect hemolymph. When the 30Kc19 protein produced from recombinant Escherichia coil was supplemented into the medium for mammalian cell culture, 30Kc19 efficiently penetrated into various types of cells and localized at subcellular compartments including mitochondria and cytoplasm. 30Kc19 also delivered cargo proteins such as green fluorescence protein into the cells even though cargo proteins are not able to penetrate into cells by themselves. In addition to the in vitro system, 30Kc19 exhibited the protein transduction property in vivo. When 30Kc19 was intraperitoneally injected into mice, 30Kc19 delivered cargo proteins into various organ tissues of model animals without producing toxicity. Therefore, 30Kc19 has a great potential as a cell-penetrating protein that can be used as a medicinal tool to deliver cargo molecules including proteins into the target organ tissues in the body. (C) 2012 Elsevier Ltd. All rights reserved.
ISSN
0142-9612
http://dx.doi.org/10.1016/j.biomaterials.2012.08.063
0142-9612
Language
English
URI
http://hdl.handle.net/10371/83293
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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