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Lentivirus-mediated carboxyl-terminal modulator protein gene transfection via aerosol in lungs of K-ras null mice

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dc.contributor.authorHwang, S-K-
dc.contributor.authorKwon, J-T-
dc.contributor.authorPark, S-J-
dc.contributor.authorChang, S-H-
dc.contributor.authorLee, E-S-
dc.contributor.authorChung, Y-S-
dc.contributor.authorJr, GR Beck-
dc.contributor.authorLee, KH-
dc.contributor.authorPiao, L-
dc.contributor.authorPark, J-
dc.contributor.authorCho, Myung-Haing-
dc.date.accessioned2009-09-01-
dc.date.available2009-09-01-
dc.date.issued2007-10-25-
dc.identifier.citationGene Ther 2007; 14: 1721-1730en
dc.identifier.issn0969-7128-
dc.identifier.urihttps://hdl.handle.net/10371/8329-
dc.description.abstractThe low efficiency of conventional therapies in achieving long-term survival of lung cancer patients calls for development of novel options. Aerosol gene delivery may provide the alternative for safe and effective treatment for lung cancer. Therefore, current study was performed to elucidate the potential effects of C-terminal modulator protein (CTMP) via aerosol on lung tumorigenesis. Lentiviral vector-CTMP was delivered into K-ras null lung cancer mice through the nose-only inhalation system for 30 min. After 48 h, the potential effects of CTMP on Akt1-related signals and cell cycle regulation in the lungs were evaluated by western blot, immunohistochemistry and zymography. Lentivirus-based CTMP delivery inhibited the Akt1 activity through selective suppression of Akt1 phosphorylation at Ser473. Aerosol delivery of CTMP inhibited proteins important for Akt1 signals, cell cycle and tumor metastasis in lungs of K-ras null mice. Together, our results suggest that lentivirus-mediated aerosol delivery of CTMP may be compatible with noninvasive in vivo gene therapy. Our results emphasize the importance of noninvasive-targeted delivery of CTMP for lung cancer therapy in the future. While the studies are conducted in mice, it is envisioned that noninvasive targeting the specific genes responsible for cancer progression is an attractive strategy for effective anticancer therapeutics.en
dc.description.sponsorshipThis work was partially supported by the grants from the
Korea Research Foundation (KRF-2006-311-E00507) in
Korea. MHC and SHC were supported by the Nano
Systems Institute-National Core Research Center (NSINCRC)
program of KOSEF. SKH, JTK, SJP, ESL, YSC are
also grateful for the award of the BK21 fellowship. KHL
was supported by 21C Frontier Functional Human
Genome Project (FG03-0601-003-1-0-0) and National
Nuclear R&D Program from Ministry of Science and
Technology. GRB Jr was supported by National Cancer
Institute Grant CA84573.
en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.subjectaerosol gene deliveryen
dc.subjectCTMPen
dc.subjectAkt1en
dc.subjectlung canceren
dc.subjectlentivirus systemen
dc.titleLentivirus-mediated carboxyl-terminal modulator protein gene transfection via aerosol in lungs of K-ras null miceen
dc.typeArticleen
dc.contributor.AlternativeAuthor조명행-
dc.identifier.doi10.1038/sj.gt.3303042-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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