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Receptor-mediated gene delivery by folate-PEG-baculovirus in vitro

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dc.contributor.authorKim, You-Kyoung-
dc.contributor.authorChoi, Jae Young-
dc.contributor.authorYoo, Mi-Kyong-
dc.contributor.authorJiang, Hu-Lin-
dc.contributor.authorArote, Rohidas-
dc.contributor.authorJe, Yeon Ho-
dc.contributor.authorCho, Myung-Haing-
dc.contributor.authorCho, Chong-Su-
dc.date.accessioned2009-09-01-
dc.date.available2009-09-01-
dc.date.issued2007-09-
dc.identifier.citationJournal of Biotechnology, Vol.131 No.3, pp.353-361-
dc.identifier.issn0168-1656-
dc.identifier.other30990-
dc.identifier.urihttps://hdl.handle.net/10371/8341-
dc.description.abstractGene delivery using baculovirus is a promising approach for efficient and safe gene therapy compared with animal viruses. However, obstacles of baculovirus-mediated gene delivery include inactivation of baculovirus in human serum and whole blood and the lack of specificity in targeted delivery. Therefore, chemical modification of the viral surface with poly(ethylene glycol) (PEG) and a targeting ligand, such as folate, is necessary for stable and targeted gene delivery via receptor-mediated endocytosis. In this study, folate-PEG (F-PEG) was attached on the baculovirus surface to obtain efficiency and specificity of gene delivery. Composition of F-PEG and degree of capsid modification with F-PEG was determined using H-1 nuclear magnetic resonance (H-1 NMR) and fluorescamine assay, respectively. Folate-PEG-Baculovirus (F-P-Bac) showed enhanced transduction efficiency compared to PEG-Baculovirus (P-Bac) in folate receptor (FR)-positive KB cells. Moreover, this enhanced transduction was not observed in FR-negative HepG2 cells. Presence of free folate in the medium blocked the transduction of F-P-Bac, whereas transduction efficiency of P-Bac in the presence or absence of free folate was not changed significantly. This study thus suggests that F-P-Bac can be used as a receptor-mediated gene delivery system. (C) 2007 Elsevier B.V. All rights reserved.-
dc.description.sponsorshipThis research work was supported by funds provided by Korea Science and Engineering Foundation (KOSEF) (R01-2005-000-10087-0). We also acknowledge the National Instrumentation Center for Environmental Management (NICEM) for permission to take NMR measurements. Y.K. Kim was supported by the BK21 grant.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectgene therapy-
dc.subjecttargeted delivery-
dc.subjectfolate receptor-
dc.subjectFolate-PEG-Baculovirus-
dc.titleReceptor-mediated gene delivery by folate-PEG-baculovirus in vitro-
dc.typeArticle-
dc.contributor.AlternativeAuthor조명행-
dc.identifier.doi10.1016/j.jbiotec.2007.07.938-
dc.citation.journaltitleJournal of Biotechnology-
dc.identifier.scopusid2-s2.0-34548409638-
dc.citation.endpage361-
dc.citation.number3-
dc.citation.startpage353-
dc.citation.volume131-
dc.identifier.rimsid30990-
dc.identifier.sci000250031300018-
dc.contributor.affiliatedAuthorCho, Myung-Haing-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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