Browse

Antiproliferation Properties of Grain Sorghum Dry Distiller's Grain Lipids in Caco-2 Cells

DC Field Value Language
dc.contributor.authorZbasnik, Richard-
dc.contributor.authorCarr, Timothy-
dc.contributor.authorWeller, Curtis-
dc.contributor.authorHwang, Keum Taek-
dc.contributor.authorWang, Lijun-
dc.contributor.authorCuppett, Susan-
dc.contributor.authorSchlegel, Vicki-
dc.creator황금택-
dc.date.accessioned2013-10-18T00:31:08Z-
dc.date.available2013-10-18T00:31:08Z-
dc.date.issued2009-10-
dc.identifier.citationJournal of Agricultural and Food Chemistry Vol.57 No.21, pp. 10435-10441-
dc.identifier.issn0021-8561-
dc.identifier.urihttps://hdl.handle.net/10371/83693-
dc.description.abstractAntiproliferative properties of lipids extracted from grain sorghum (GS) dry distillers grain (DDG) were analyzed to determine the feasibility of developing GS coproducts as a source for human health dietary ingredients. The lipid extract of GS-DDG was delivered to human colon carcinoma (Caco-2) cells by solubilizing 0−1000 μg/mL of GS-DDG lipids in 100 μg/mL increments with micelles. A significant reduction in cell viability (25−50%) resulted at treatment levels of 400−1000 μg/mL GS-DDG lipids (p < 0.05). Alternatively, total protein levels of cells treated with 400, 500, and 600 μg/mL of GS-DDG lipid were not significantly different from the control, indicating cell growth during the treatment period. Total cell counts for the control were not significantly different from the GS-DDG lipid treated cells, but dead cell counts increased by 10% for the latter sample with a concomitant increase of the intercellular protein lactate dehydrogenase leakage (30−40%) in the medium. Preliminary analysis by the fluorescence-activated cell method (FACs) demonstrated that nonviable cells were in either the early apoptotic, late apoptotic, or necrotic stage post-treatment with 400, 500, and 600 μg/mL GS-DDG lipids. Physiochemical characterization of the GS-DDG lipids used for the antiproliferation study showed the presence of vitamin E (predominantly γ-tocopherol), triacylglycerides (predominantly linoleic acid), policosanols, aldehydes, and sterols (predominantly campesterol and stigmasterol), each of which or as synergistic/additive group of constituents may be responsible for the antiproliferative effect.-
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.subject복합학en
dc.subjectGrain sorghum-
dc.subjectantiproliferation-
dc.subjectethanol-
dc.subjectdry distiller’s grain-
dc.subjectCaco-2 cells-
dc.titleAntiproliferation Properties of Grain Sorghum Dry Distiller's Grain Lipids in Caco-2 Cellsen
dc.typeArticle-
dc.author.alternative황금택-
dc.identifier.doi10.1021/jf902136p-
dc.citation.journaltitleJournal of Agricultural and Food Chemistry-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2009-01/102/0000050589/6-
dc.description.srndSEQ:6-
dc.description.srndPERF_CD:SNU2009-01-
dc.description.srndEVAL_ITEM_CD:102-
dc.description.srndUSER_ID:0000050589-
dc.description.srndADJUST_YN:N-
dc.description.srndEMP_ID:A077289-
dc.description.srndDEPT_CD:352-
dc.description.srndCITE_RATE:2.469-
dc.description.srndFILENAME:첨부된 내역이 없습니다.-
dc.description.srndDEPT_NM:식품영양학과-
dc.description.srndEMAIL:keum@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.srnd2009-01/102/0000050589/6-
Appears in Collections:
College of Human Ecology (생활과학대학)Dept. of Food and Nutrition (식품영양학과)Journal Papers (저널논문_식품영양학과)
Files in This Item:
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse