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Antiproliferation Properties of Grain Sorghum Dry Distiller's Grain Lipids in Caco-2 Cells
DC Field | Value | Language |
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dc.contributor.author | Zbasnik, Richard | - |
dc.contributor.author | Carr, Timothy | - |
dc.contributor.author | Weller, Curtis | - |
dc.contributor.author | Hwang, Keum Taek | - |
dc.contributor.author | Wang, Lijun | - |
dc.contributor.author | Cuppett, Susan | - |
dc.contributor.author | Schlegel, Vicki | - |
dc.creator | 황금택 | - |
dc.date.accessioned | 2013-10-18T00:31:08Z | - |
dc.date.available | 2013-10-18T00:31:08Z | - |
dc.date.issued | 2009-10 | - |
dc.identifier.citation | Journal of Agricultural and Food Chemistry Vol.57 No.21, pp. 10435-10441 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.uri | https://hdl.handle.net/10371/83693 | - |
dc.description.abstract | Antiproliferative properties of lipids extracted from grain sorghum (GS) dry distillers grain (DDG) were analyzed to determine the feasibility of developing GS coproducts as a source for human health dietary ingredients. The lipid extract of GS-DDG was delivered to human colon carcinoma (Caco-2) cells by solubilizing 0−1000 μg/mL of GS-DDG lipids in 100 μg/mL increments with micelles. A significant reduction in cell viability (25−50%) resulted at treatment levels of 400−1000 μg/mL GS-DDG lipids (p < 0.05). Alternatively, total protein levels of cells treated with 400, 500, and 600 μg/mL of GS-DDG lipid were not significantly different from the control, indicating cell growth during the treatment period. Total cell counts for the control were not significantly different from the GS-DDG lipid treated cells, but dead cell counts increased by 10% for the latter sample with a concomitant increase of the intercellular protein lactate dehydrogenase leakage (30−40%) in the medium. Preliminary analysis by the fluorescence-activated cell method (FACs) demonstrated that nonviable cells were in either the early apoptotic, late apoptotic, or necrotic stage post-treatment with 400, 500, and 600 μg/mL GS-DDG lipids. Physiochemical characterization of the GS-DDG lipids used for the antiproliferation study showed the presence of vitamin E (predominantly γ-tocopherol), triacylglycerides (predominantly linoleic acid), policosanols, aldehydes, and sterols (predominantly campesterol and stigmasterol), each of which or as synergistic/additive group of constituents may be responsible for the antiproliferative effect. | - |
dc.language.iso | en | en |
dc.publisher | American Chemical Society | en |
dc.subject | 복합학 | en |
dc.subject | Grain sorghum | - |
dc.subject | antiproliferation | - |
dc.subject | ethanol | - |
dc.subject | dry distiller’s grain | - |
dc.subject | Caco-2 cells | - |
dc.title | Antiproliferation Properties of Grain Sorghum Dry Distiller's Grain Lipids in Caco-2 Cells | en |
dc.type | Article | - |
dc.author.alternative | 황금택 | - |
dc.identifier.doi | 10.1021/jf902136p | - |
dc.citation.journaltitle | Journal of Agricultural and Food Chemistry | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2009-01/102/0000050589/6 | - |
dc.description.srnd | SEQ:6 | - |
dc.description.srnd | PERF_CD:SNU2009-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000050589 | - |
dc.description.srnd | ADJUST_YN:N | - |
dc.description.srnd | EMP_ID:A077289 | - |
dc.description.srnd | DEPT_CD:352 | - |
dc.description.srnd | CITE_RATE:2.469 | - |
dc.description.srnd | FILENAME:첨부된 내역이 없습니다. | - |
dc.description.srnd | DEPT_NM:식품영양학과 | - |
dc.description.srnd | EMAIL:keum@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | 2009-01/102/0000050589/6 | - |
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